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Type: Artigo de periódico
Title: A Role for galectin-3 in renal tissue damage triggered by ischemia and reperfusion injury
Author: BERTOCCHI, Ana Paula Fernandes
WANG, Pamella Huey Mei
GONCALVES, Giselle Martins
DAMIAO, Marcio Jose
CENEDEZE, Marcos Antonio
BERALDO, Felipe Caetano
TEIXEIRA, Vicente de Paula Antunes
REIS, Marlene Antonia dos
MAZZALI, Marilda
CAMARA, Niels Olsen Saraiva
Abstract: Ischemic-reperfusion injury (IRI) triggers an inflammatory response involving neutrophils/macrophages, lymphocytes and endothelial cells. Galectin-3 is a multi-functional lectin with a broad range of action such as promotion of neutrophil adhesion, induction of oxidative stress, mastocyte migration and degranulation, and production of pro-inflammatory cytokines. The aim of this study was evaluate the role of galectin-3 in the inflammation triggered by IRI. Galectin-3 knockout (KO) and wild type (wt) mice were subjected to 45 min of renal pedicle occlusion. Blood and kidney samples were collected at 6, 24, 48 and 120 h. Blood urea was analyzed enzymatically, while MCP-1, IL-6 and IL-1 beta were studied by real-time PCR. Reactive oxygen species (ROS) was investigated by flow cytometry. Morphometric analyses were performed at 6, 24, 48 and 120 h after reperfusion. Urea peaked at 24 h, being significantly lower in knockout animals (wt = 264.4 +/- 85.21 mg/dl vs. gal-3 KO = 123.74 +/- 29.64 mg/dl, P = 0.001). Galectin-3 knockout animals presented less acute tubular necrosis and a more prominent tubular regeneration when compared with controls concurrently with lower expression of MCP-1, IL-6, IL-1 beta, less macrophage infiltration and lower ROS production at early time points. Galectin-3 seems to play a role in renal IRI involving the secretion of macrophage-related chemokine, pro-inflammatory cytokines and ROS production.
Subject: galectin-3
IL-1 beta
ischemia and reperfusion injury
Country: Estados Unidos
Citation: TRANSPLANT INTERNATIONAL, v.21, n.10, p.999-1007, 2008
Rights: fechado
Identifier DOI: 10.1111/j.1432-2277.2008.00705.x
Date Issue: 2008
Appears in Collections:FCM - Artigos e Outros Documentos

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