Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/95642
Type: Artigo de periódico
Title: Effect Of Fasting On Insulin Signaling In The Aorta Of Intact Rats
Author: Silva L.A.C.V.
Carvalho C.R.O.
Saad M.J.A.
Abstract: Insulin stimulates the tyrosine kinase activity of its receptor, resulting in the phosphorylation of its cytosolic substrate, insulin receptor substrate 1 (IRS-1). Previous studies have demonstrated a tissue-specific regulation of IRS-1. In the present study we investigated the levels and phosphorylation state of IRS-1 after insulin stimulation in the rat aorta in vivo, and the modulation of this protein after 72 h of fasting, using immunoprecipitation and immunoblotting with anti-insulin receptor, anti-IRS-1 and antiphosphotyrosine antibodies. We show that IRS-1 is present in rat aorta, and is tyrosine phosphorylated after insulin stimulation. After insulin stimulation, rats fasted for 72 h showed an increase in insulin receptor (100 ± 45%, P<0.05) and IRS-1 phosphorylation (68 ±24%, P±0.05) in aorta, compared to fed rats. There was no change in insulin receptor or IRS-1 protein levels in fasted rats. In summary, the present study demonstrated that proteins involved in the early steps of insulin signal transduction are present in the rat aorta and can be modulated by fasting. It will be of interest to study the regulation of these proteins in the aorta of animal models of hypertension and/or atherosclerosis.
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Rights: aberto
Identifier DOI: 
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-0030324947&partnerID=40&md5=79252549bc9ab768c1d48aa2958461a9
Date Issue: 1996
Appears in Collections:Unicamp - Artigos e Outros Documentos

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