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Type: Artigo de periódico
Title: Peg + Potassium Phosphate + Urea Aqueous Two-phase Systems: Phase Equilibrium And Protein Partitioning
Author: Da Silva L.H.M.
Meirelles A.J.A.
Abstract: Liquid-liquid equilibria of poly(ethylene glycol) (PEG) + potassium phosphate + urea + water aqueous two-phase systems were studied for different PEG molecular weights and pHs. Experimental techniques and analytical methods are described. Equilibrium data were obtained for PEG (1450, 3350, 10000) + potassium phosphate (pH 7 and 9) + urea (6 mass%) + water aqueous two-phase systems at 25°C. Four tie lines were measured for each system. The partitioning behaviors of lysozyme, catalase, and β-galactosidase were studied in these systems at 25°C, at pH 7, and at the urea concentrations 3 and 6 mass %.
Rights: fechado
Identifier DOI: 10.1021/je000131f
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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