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Type: Artigo de periódico
Title: Isolation And Characterization Of Isolectins From Talisia Esculenta Seeds
Author: Freire M.D.G.M.
Machado O.L.T.
Smolka M.B.
Marangoni S.
Novello J.C.
Macedo M.L.R.
Abstract: Four isolectins (TEL-I, TEL-II, TEL-III and TEL-IV) were isolated from seeds of Talisia esculenta by reverse-phase high-performance liquid chromatography. RP-HPLC was performed on a u-Bondapack C18 column (0.78 cm X 30 cm) (Waters 991-PDA system) at room temperature. Rechromatography of the four fractions on a C18 column under the same conditions yielded lectins with two dissimilar subunits (M r 20 kDa and 40 kDa) bound noncovalently. The isolectins showed very similar characteristics, such as molecular masses, N-terminal sequences, and hemagglutinating activity, but differed in their isoelectric points and in inhibition by carbohydrates. © 2001 Plenum Publishing Corporation.
Citation: Protein Journal. , v. 20, n. 6, p. 495 - 500, 2001.
Rights: fechado
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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