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Type: Artigo de periódico
Title: Low Protein Diet Confers Resistance To The Inhibitory Effects Of Interleukin 1β On Insulin Secretion In Pancreatic Islets
Author: Vieira E.C.
Carneiro E.M.
Latorraca M.Q.
Delguingaro-Augusto V.
Amaral M.E.C.
Bosqueiro J.R.
Boschero A.C.
Abstract: High protein content in the diet during childhood and adolescence has been associated to the onset insulin-dependent diabetes mellitus. We investigated the effect of interleukin-1β (IL-1β) on insulin secretion, glucose metabolism, and nitrite formation by islets isolated from rats fed with normal protein (NP, 17%) or low protein (LP, 6%) after weaning. Pretreatment of islets with IL-1β for 1 h or 24 h inhibited the insulin secretion induced by glucose in both groups, but it was less marked in LP than in NP group. Islets from LP rats exhibited a decreased IL-1β-induced nitric oxide (NO) production, lower inhibition of D-[U14C]-glucose oxidation to 14CO2 and less pronounced effect of IL-1β on α-ketoisocaproic acid-induced insulin secretion than NP islets. However, when the islets were stimulated by high concentrations of K+ the inhibitory effect of IL-1β on insulin secretion was not different between groups. In conclusion, protein restriction protects β-cells of the deleterious effect of IL-1β, apparently, by decreasing NO production. The lower NO generation in islets from protein deprived rats may be due to increased free fatty acids oxidation and consequent alteration in Ca2+ homeostasis. © 2001 Elsevier Science Inc.
Rights: fechado
Identifier DOI: 10.1016/S0955-2863(01)00140-1
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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