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Type: Artigo de periódico
Title: Isolation And Characterization Of A Convulxin-like Protein From Crotalus Durissus Collilineatus Venom
Author: Toyama M.H.
Carneiro E.M.
Marangoni S.
Amaral M.E.C.
Velloso L.A.
Boschero A.C.
Abstract: A convulxin (Cvx)-like protein was isolated from Crotalus durissus collilineatus venom by a combination of molecular exclusion and reversed-phase HPLC chromatographies. The molecular mass of the Cvx-like protein in the absence and presence of DTT was 78 kDa and 12-13 kDa, respectively. The Cvx-like protein consisted of two nonidentical polypeptide chains (α and β). The N-terminal amino-acid sequences of the α and β subunits were GLHCPSDWYAYDGHCYKIFNEEMNWED and GFCCPSHWSSYSRYCYKFFSQEMNWEDAEK, respectively, with both subunits having a high content of Glu, Ser, Cys, and Asp. The Cvx-like protein showed high homology with other venom C-type lectins, but had low hemagglutinating activity on intact and trypsinized erythrocytes. The Cvx-like protein stimulated insulin receptor phosphorylation and potentiated insulin secretion from isolated islets in the presence of sub- (2.8 mM) or supra-physiological (16.7 mM) glucose concentrations. These results suggest that the increase in insulin secretion induced by Cvx-like protein may be mediated by a protein tyrosine kinase-dependent pathway and may involve other membrane receptors, such as GP VI or Scr family proteins.
Citation: Journal Of Protein Chemistry. , v. 20, n. 7, p. 585 - 591, 2001.
Rights: fechado
Identifier DOI: 10.1023/A:1013377331569
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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