Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/94694
Type: Artigo de periódico
Title: Expression Of α-hemoglobin Stabilizing Protein Gene During Human Erythropoiesis
Author: Dos Santos C.O.
Duarte A.S.S.
Olalla Saad S.T.
Costa F.F.
Abstract: α-Hemoglobin stabilizing protein (AHSP) is an abundant, erythroid-specific protein that forms a stable complex with free α-hemoglobin but not with β-hemoglobin or hemoglobin A. As such, AHSP is required for normal erythropoiesis, probably acting by blocking the deleterious effects of free α-hemoglobin precipitation. In order to study the levels of expression of the AHSP gene during the different phases of erythropoiesis, we carried out a two-phase liquid culture of erythroid cells and real-time quantitative polymerase chain reaction. Blood from control volunteers was cultured with erythropoietin to stimulate differentiation. The different stages of erythropoiesis were confirmed by morphologic and flow cytometric analysis. The results showed a progressive increase in AHSP gene expression following the expression of α-globin gene, during maturation of the red blood cell precursors, confirming the probable important function of this protein during normal erythropoiesis. © 2004 International Society for Experimental Hematology. Published by Elsevier Inc.
Editor: 
Rights: fechado
Identifier DOI: 10.1016/j.exphem.2003.11.002
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-0842287506&partnerID=40&md5=93b285df7a2f64bdee07093523fa0716
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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