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Type: Artigo de evento
Title: Rapid Method For Evaluating Reversed-phase High-performance Liquid Chromatography Column Stability
Author: Fonseca D.A.
Gutierrez H.R.
Collins K.E.
Collins C.H.
Abstract: A procedure is presented for the rapid evaluation of HPLC stationary phase stability at pH 8.4 or 10.1 using a temperature of 60°C. Mobile phase (MeOH-0.1moll-1 aqueous NaHCO3, 50:50, v/v) is continuously passed through the column with periodic injections of a test solution until the several chromatographic parameters of the resulting chromatograms are degraded. The tests were applied to several commercial and laboratory-made stationary phases. After degradation two of these phases, one commercial and one laboratory-made, were examined by elemental analysis and scanning electron microscopy to elucidate the degradation process. © 2004 Elsevier B.V. All rights reserved.
Rights: fechado
Identifier DOI: 10.1016/j.chroma.2003.12.033
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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