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Type: Artigo de evento
Title: Effect Of Ingap (islet Neogenesis Associated Protein) Upon Insulin Secretion Of Pancreatic Islets From Adults And Neonatal Rats [efecto Del Ingap (proteína Asociada A La Neogénesis Insular) Sobre La Secreción De Insulina De Islotes Pancreáticos De Ratas Adultas Y Recién Nacidas]
Author: Borelli M.I.
Stoppiglia L.F.
Rezende L.F.
Flores L.E.
Del Zotto H.
Boschero A.C.
Gagliardino J.J.
Abstract: Aim: This study was undertaken to determine the effect of 15aa-INGAP upon insulin secretion. Material and methods: Isolated islets (collagenase) from normal male adult or neonatal Wistar rats were cultured (4 days) in RPMI 1640 medium, in the presence of 10mM glucose with or without the addition of 15aa-INGAP to the medium (30μM). After culture, the islets were incubated during 90 min in the presence of 2.8, 8.3 or 16.7 mM glucose, of 8.3 mM glucose plus 10mM leucine or arginine. Insulin was measured in the incubation medium by radioimmunoassay. Results: Insulin secretion of islets from either neonatal or adult rats was significantly enhanced in the presence of 15aa-INGAP in the culture media in response to glucose or other secretagogues (leucine and arginine). Islets from adult rats released significantly larger amounts of insulin than those from newborn rats. Conclusions: Our results demonstrate for the first time that INGAP enhanced the β-cell response to glucose and aminoacids. Therefore, INGAP would represent a treatment alternative for patients with diminished insulin secretion.
Rights: aberto
Identifier DOI: 
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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