Please use this identifier to cite or link to this item:
|Type:||Artigo de periódico|
|Title:||Aluminium Triggers Necrosis And Apoptosis In V79 Cells|
|Abstract:||The aim of this work was to examine the induction of cell death by aluminium (Al) at low pH, where the toxic cation Al3+ has maximum activity. Chinese hamster V79 fibroblasts were used as a model to study the toxic effects of different Al concentrations under optimal conditions for Al3+ activity (pH 4.5). Al3+ was detected by 8-hydroxyquinoline staining and cell death was assessed by image analysis, TUNEL assay and annexin-V labelling. Intracellular Al3+ deposits occurred mainly in the cytoplasm and within the nucleus. Al3+ altered the nuclear morphology, with chromatin condensation and loss being observed in Al3+-treated cells. Necrosis was detected with 10-5- 10-3 M AlCl3 and apoptosis was observed only at 10 -8-10-5 M AlCl3. Our results show that Al 3+ induces cell death through a mechanism which could determine whether necrosis or apoptosis would be triggered.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.