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Type: Artigo de evento
Title: Microspectroscopy And Scanning Microscopy In An Optical Tweezers System
Author: Fontes A.
Neves A.A.R.
Moreira W.L.
De Thomaz A.A.
Barbosa L.C.
De Parias P.M.A.
Santos B.S.
Ferreira R.C.
De Paula A.M.
Ajito K.
Cesar C.L.
Abstract: In this work we developed a setup consisting of an Optical Tweezers equipped with linear and non-linear microspectroscopy system to add the capabilities of manipulation and analysing captured objects. Our setup includes a homemade confocal spectrometer using a monochromator equipped with a liquid nitrogen cooled CCD. The spectroscopic laser system included a cw and a femtosecond Ti:sapphire lasers that allowed us to perform Raman, hyper-Raman, hyper-Rayleigh and two photon Excited (TPE) luminescence in particles trapped with an Nd:YAG cw laser, We obtained Raman spectra of a single trapped polystyrene microsphere and a single trapped red blood cell to evaluate the performance of our system. We also observed hyper-Rayleigh and hyper-Raman peaks for SrTiO3 with 60s integration time only. This was possible because the repetition rate of the femtosecond Ti:sapphire lasers, on the order of 80 MHz, are much higher than the few kHz typical picosecond laser repetition rate used before in hyper-Raman experiment, which required acquisition times of order of few hours. We used this system to perform scanning microscopy and to acquire TPE luminescence spectra of captured single stained microsphere and cells conjugated with quantum dots of CdS and CdTe and hyper-Rayleigh spectra of a noncaptured ZnSe microparticle. The results obtained show the potential presented by this system and fluorescent labels to perform spectroscopy in a living trapped microorganism in any neighbourhood and dynamically observe the chemical reactions changes in real time.
Rights: aberto
Identifier DOI: 10.1117/12.620754
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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