Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/93802
Type: Artigo de evento
Title: Mechanical Properties Of Stored Red Blood Cells Using Optical Tweezers
Author: Fontes A.
De Thomaz A.A.
De Pozzo L.Y.
De Barjas-Castro M.L.
Brandao M.M.
Saad S.T.O.
Barbosa L.C.
Cesar C.L.
Abstract: We have developed a method for measuring the red blood cell (RBC) membrane overall elasticity μ by measuring the deformation of the cells when dragged at a constant velocity through a plasma fluid by an optical tweezers. The deformability of erythrocytes is a critical determinant of blood flow in the microcirculation. We tested our method and hydrodynamic models, which included the presence of two walls, by measuring the RBC deformation as a function of drag velocity and of the distance to the walls. The capability and sensitivity of this method can be evaluated by its application to a variety of studies, such as, the measurement of RBC elasticity of sickle cell anemia patients comparing homozygous (HbSS), including patients taking hydroxyrea (HU) and heterozygous (HbAS) with normal donors and the RBC elasticity measurement of gamma irradiated stored blood for transfusion to immunosupressed patients as a function of time and dose. These studies show that the technique has the sensitivity to discriminate heterozygous and homozygous sickle cell anemia patients from normal donors and even follow the course of HU treatment of Homozygous patients. The gamma irradiation studies show that there is no significant change in RBC elasticity over time for up to 14 days of storage, regardless of whether the unit was irradiated or not, but there was a huge change in the measured elasticity for the RBC units stored for more than 21 days after irradiation. These finds are important for the assessment of stored irradiated RBC viability for transfusion purposes because the present protocol consider 28 storage days after irradiation as the limit for the RBC usage.
Editor: 
Rights: aberto
Identifier DOI: 10.1117/12.620758
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-31844432663&partnerID=40&md5=79dfb0979046dddf2312a4d3111befab
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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