Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/92982
Type: Artigo de periódico
Title: Production, Purification And Application Of Extracellular Chitinase From Cellulosimicrobium Cellulans 191 [produção, Purificação E Aplicação Da Quitinase Extracelular De Cellulosimicrobium Cellulans 191]
Author: Fleuri L.F.
Kawaguti H.Y.
Sato H.H.
Abstract: This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25°C and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25°C and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts.
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Rights: aberto
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Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-70349507282&partnerID=40&md5=0f9ced562d8095a446870099889310bc
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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