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|Type:||Artigo de periódico|
|Title:||Pip4kiia And β-globin: Transcripts Differentially Expressed In Reticulocytes And Associated With High Levels Of Hb H In Two Siblings With Hb H Disease|
|Abstract:||We are reporting here the results of differential gene expression experiments comparing two siblings, a 21-yr-old male and a 19-yr-old female, with the same alpha-thalassemia genotype (-α 3.7/ -SEA) and quite different levels of Hb H in the peripheral blood (18.7 and 5%, respectively). By using mRNA differential-display reverse-transcription-PCR and suppression subtractive hybridization, two main transcripts were selected in both procedures and validated by qRT-PCR, one corresponding to the phosphatidylinositol phosphate 4-kinase type II-alpha (PIP4KIIA) gene and the other to the β-globin gene, both over expressed in the patient with the higher percentage of Hb H. Type II PIP kinases produce phosphatidylinositol 4,5 biphosphate, a critical and pleiotropic regulatory molecule involved in diverse cellular activities, including gene expression. Our results suggest that PIP4KIIA may be one of the factors related to the regulation of the β-globin gene expression and the different levels of Hb H in α-thalassemic patients. © 2009 John Wiley & Sons A/S.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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