Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/92671
Type: Artigo de periódico
Title: Pip4kiia And β-globin: Transcripts Differentially Expressed In Reticulocytes And Associated With High Levels Of Hb H In Two Siblings With Hb H Disease
Author: Wenning M.R.S.C.
Mello M.P.
Andrade T.G.
Lanaro C.
Albuquerque D.M.
Saad S.T.O.
Costa F.F.
Sonati M.F.
Abstract: We are reporting here the results of differential gene expression experiments comparing two siblings, a 21-yr-old male and a 19-yr-old female, with the same alpha-thalassemia genotype (-α 3.7/ -SEA) and quite different levels of Hb H in the peripheral blood (18.7 and 5%, respectively). By using mRNA differential-display reverse-transcription-PCR and suppression subtractive hybridization, two main transcripts were selected in both procedures and validated by qRT-PCR, one corresponding to the phosphatidylinositol phosphate 4-kinase type II-alpha (PIP4KIIA) gene and the other to the β-globin gene, both over expressed in the patient with the higher percentage of Hb H. Type II PIP kinases produce phosphatidylinositol 4,5 biphosphate, a critical and pleiotropic regulatory molecule involved in diverse cellular activities, including gene expression. Our results suggest that PIP4KIIA may be one of the factors related to the regulation of the β-globin gene expression and the different levels of Hb H in α-thalassemic patients. © 2009 John Wiley & Sons A/S.
Editor: 
Rights: fechado
Identifier DOI: 10.1111/j.1600-0609.2009.01325.x
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-70349944006&partnerID=40&md5=f0ed0cb24bd8379824a68b958a751b7c
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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