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Type: Artigo de periódico
Title: Islet Neogenesis-associated Protein Pentadecapeptide (ingap-pp): Mechanisms Involved In Its Effect Upon β-cell Mass And Function
Author: Madrid V.
Del Zotto H.
Maiztegui B.
Raschia M.A.
Alzugaray M.E.
Boschero A.C.
Barbosa H.C.
Flores L.E.
Borelli M.I.
Gagliardino J.J.
Abstract: The effect of islet neogenesis-associated protein pentadecapeptide (INGAP-PP) administration to normal male hamsters upon serum glucose and triglyceride levels, β-cell mass and function was studied. INGAP-PP (500 μg) or saline was injected twice daily during 10 days. Both groups showed comparable body weight, serum glucose and triglyceride levels. INGAP-PP treated animals had significantly higher HOMA-IR and HOMA-β and their islets released more insulin in response to glucose; they had lower islet DNA content, significantly increased number of islets/unit area, β-cell replication rate and mass, cells co-expressing Pdx-1/INGAP and islets in contact with ducts, and decreased β-cell apoptosis rate. The percentage of cells expressing Pdx-1 alone or together with INGAP or insulin increased significantly in ducts. These animals also showed a significantly higher concentration of Pdx-1 and Ngn-3 mRNA and a lower number of INGAP-positive cells. In conclusion, INGAP-PP promoted a controlled and functionally active increase of β-cell mass; our data demonstrate for the first time the mechanism responsible for such changes; that Ngn-3 would be involved in INGAP-PP-induced neogenesis; and the existence of a negative feedback loop with endogenous INGAP-producing cells. Accordingly, INGAP-PP could be used to induce these effects in people with or at risk of developing diabetes. © 2009 Elsevier B.V. All rights reserved.
Rights: fechado
Identifier DOI: 10.1016/j.regpep.2009.05.011
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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