Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/92455
Type: Artigo de periódico
Title: Reactive Oxygen Species Generation In Peripheral Blood Monocytes And Oxidized Ldl Are Increased In Hyperlipidemic Patients
Author: Vasconcelos E.M.A.
Degasperi G.R.
de Oliveira H.C.F.
Vercesi A.E.
de Faria E.C.
Castilho L.N.
Abstract: Objectives: Experimental and in vitro evidences have established that reactive oxygen species (ROS) generated by vascular wall cells play a key role in atherogenesis. Here, we evaluated the rate of ROS generation by resting peripheral monocytes in naive hyperlipidemic subjects. Design and methods: Primary hypercholesterolemic, combined hyperlipidemic, and normolipidemic individuals were studied. ROS generation and the mitochondrial electrical transmembrane potential were estimated by flow cytometry. Plasma oxidized (ox) LDL levels and lipid profile were measured by ELISA and enzymatic colorimetric methods. Results: Both hyperlipidemic groups presented significantly higher rates of monocyte ROS generation and elevated plasma levels of ox-LDL. Combined hyperlipidemic subjects presented increased levels of small dense LDL and insulin. Significant positive correlations between monocyte ROS generation and ox-LDL concentrations were found in pooled data. Conclusions: These data provide evidence that ROS production by circulating monocytes from hyperlipidemic subjects may contribute to the systemic oxidative stress and possibly to atherogenesis. © 2009 Elsevier Inc. All rights reserved.
Editor: 
Rights: fechado
Identifier DOI: 10.1016/j.clinbiochem.2009.05.010
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-67650753558&partnerID=40&md5=57bad26dc0faf3c3c01304063d3472b0
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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