Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/92198
Type: Artigo de periódico
Title: Immobilized Metal-ion Affinity Chromatography (imac) Of Biomolecules: Fundamental Aspects And Technological Applications [cromatografia De Afinidade Por Íons Metálicos Imobilizados (imac) De Biomoléculas: Aspectos Fundamentais E Aplicações Tecnológicas]
Author: Bresolin I.T.L.
Miranda E.A.
Bueno S.M.A.
Abstract: Immobilized Metal Ion Affinity Cromatography - IMAC - is a group-specific based adsorption applied to the purification and structure-function studies of proteins and nucleic acids. The adsorption is based on coordination between a metal ion chelated on the surface of a solid matrix and electron donor groups at the surface of the biomolecule. IMAC is a highly selective, low cost, and easily scaled-up technique being used in research and commercial operations. A separation process can be designed for a specific molecule by just selecting an appropriate metal ion, chelating agent, and operational conditions such as pH, ionic strength, and buffer type.
Editor: 
Rights: aberto
Identifier DOI: 
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-69249177114&partnerID=40&md5=da5634e2fc4110082daa01b581e2e006
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
2-s2.0-69249177114.pdf346.01 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.