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|Type:||Artigo de periódico|
|Title:||Immobilized Metal-ion Affinity Chromatography (imac) Of Biomolecules: Fundamental Aspects And Technological Applications [cromatografia De Afinidade Por Íons Metálicos Imobilizados (imac) De Biomoléculas: Aspectos Fundamentais E Aplicações Tecnológicas]|
|Abstract:||Immobilized Metal Ion Affinity Cromatography - IMAC - is a group-specific based adsorption applied to the purification and structure-function studies of proteins and nucleic acids. The adsorption is based on coordination between a metal ion chelated on the surface of a solid matrix and electron donor groups at the surface of the biomolecule. IMAC is a highly selective, low cost, and easily scaled-up technique being used in research and commercial operations. A separation process can be designed for a specific molecule by just selecting an appropriate metal ion, chelating agent, and operational conditions such as pH, ionic strength, and buffer type.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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