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Type: Artigo de periódico
Title: Immobilized Metal-ion Affinity Chromatography (imac) Of Biomolecules: Fundamental Aspects And Technological Applications [cromatografia De Afinidade Por Íons Metálicos Imobilizados (imac) De Biomoléculas: Aspectos Fundamentais E Aplicações Tecnológicas]
Author: Bresolin I.T.L.
Miranda E.A.
Bueno S.M.A.
Abstract: Immobilized Metal Ion Affinity Cromatography - IMAC - is a group-specific based adsorption applied to the purification and structure-function studies of proteins and nucleic acids. The adsorption is based on coordination between a metal ion chelated on the surface of a solid matrix and electron donor groups at the surface of the biomolecule. IMAC is a highly selective, low cost, and easily scaled-up technique being used in research and commercial operations. A separation process can be designed for a specific molecule by just selecting an appropriate metal ion, chelating agent, and operational conditions such as pH, ionic strength, and buffer type.
Rights: aberto
Identifier DOI: 
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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