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|Type:||Artigo de periódico|
|Title:||Multivariate Optimization And Validation Of An Analytical Methodology By Rp-hplc For The Determination Of Losartan Potassium In Capsules|
de Araujo M.B.
|Abstract:||This paper describes the optimization and validation of an analytical methodology for the determination of losartan potassium in capsules by HPLC using 25-1 fractional factorial and Doehlert designs. This multivariate approach allows a considerable improvement in chromatographic performance using fewer experiments, without additional cost for columns or other equipment. The HPLC method utilized potassium phosphate buffer (pH 6.2; 58 mmol L-1)-acetonitrile (65:35, v/v) as the mobile phase, pumped at a flow rate of 1.0 mL min-1. An octylsilane column (100 mm × 4.6 mm i.d., 5 μm) maintained at 35 °C was used as the stationary phase. UV detection was performed at 254 nm. The method was validated according to the ICH guidelines, showing accuracy, precision (intra-day relative standard deviation (R.S.D.) and inter-day R.S.D values <2.0%), selectivity, robustness and linearity (r = 0.9998) over a concentration range from 30 to 70 mg L-1 of losartan potassium. The limits of detection and quantification were 0.114 and 0.420 mg L-1, respectively. The validated method may be used to quantify losartan potassium in capsules and to determine the stability of this drug. © 2009 Elsevier B.V. All rights reserved.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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