Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/92129
Type: Artigo de periódico
Title: Multivariate Optimization And Validation Of An Analytical Methodology By Rp-hplc For The Determination Of Losartan Potassium In Capsules
Author: Bonfilio R.
Tarley C.R.T.
Pereira G.R.
Salgado H.R.N.
de Araujo M.B.
Abstract: This paper describes the optimization and validation of an analytical methodology for the determination of losartan potassium in capsules by HPLC using 25-1 fractional factorial and Doehlert designs. This multivariate approach allows a considerable improvement in chromatographic performance using fewer experiments, without additional cost for columns or other equipment. The HPLC method utilized potassium phosphate buffer (pH 6.2; 58 mmol L-1)-acetonitrile (65:35, v/v) as the mobile phase, pumped at a flow rate of 1.0 mL min-1. An octylsilane column (100 mm × 4.6 mm i.d., 5 μm) maintained at 35 °C was used as the stationary phase. UV detection was performed at 254 nm. The method was validated according to the ICH guidelines, showing accuracy, precision (intra-day relative standard deviation (R.S.D.) and inter-day R.S.D values <2.0%), selectivity, robustness and linearity (r = 0.9998) over a concentration range from 30 to 70 mg L-1 of losartan potassium. The limits of detection and quantification were 0.114 and 0.420 mg L-1, respectively. The validated method may be used to quantify losartan potassium in capsules and to determine the stability of this drug. © 2009 Elsevier B.V. All rights reserved.
Editor: 
Rights: fechado
Identifier DOI: 10.1016/j.talanta.2009.06.060
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-70349230820&partnerID=40&md5=b32033eae98eda2afcb0fae1adfc40fd
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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