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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt
dc.subject.otherMELANIN-CONCENTRATING HORMONEen
dc.subject.otherENERGY-BALANCEen
dc.subject.otherBODY-WEIGHTen
dc.subject.otherFOOD-INTAKEen
dc.subject.otherG-PROTEINen
dc.subject.otherCOLD-EXPOSUREen
dc.subject.otherALPHA-SUBUNITen
dc.subject.otherRAT-BRAINen
dc.subject.otherRECEPTORSen
dc.subject.otherOBESITYen
dc.typeArtigo de periódicopt
dc.type.categoryoriginal articlept
dc.type.versionpublishedVersionen
dc.titleDistinct subsets of hypothalamic genes are modulated by two different thermogenesis-inducing stimulipt
dc.contributor.authorSOUZA, Claudio T. Dept
dc.contributor.authorPEREIRA-DA-SILVA, Marciopt
dc.contributor.authorARAUJO, Eliana P.pt
dc.contributor.authorMORARI, Joseanept
dc.contributor.authorALVAREZ-ROJAS, Fernandapt
dc.contributor.authorBORDIN, Silvanapt
dc.contributor.authorMOREIRA-FILHO, Djalma C.pt
dc.contributor.authorCARVALHEIRA, Jose B.pt
dc.contributor.authorSAAD, Mario J.pt
dc.contributor.authorVELLOSO, Licio A.pt
unicamp.authorSOUZA, Claudio T. De:Universidade Estadual de Campinas, Faculdade de Ciências Médicas, Departamento de Medicina Internapt
unicamp.authorPEREIRA-DA-SILVA, Marcio:Universidade Estadual de Campinas, Faculdade de Ciências Médicas, Departamento de Medicina Internapt
unicamp.authorARAUJO, Ept
unicamp.author.externalBORDIN, Silvanapt
dc.subject.wosEndocrinology & Metabolismpt
dc.subject.wosNutrition & Dieteticspt
dc.description.abstractObesity results from an imbalance between food intake and energy expenditure, two vital functions that are tightly controlled by specialized neurons of the hypothalamus. The complex mechanisms that integrate these two functions are only beginning to be deciphered. The objective of this study was to determine the effect of two thermogenesis-inducing conditions, i.e., ingestion of a high-fat (HF) diet and exposure to cold environment, on the expression of 1,176 genes in the hypothalamus of Wistar rats. Hypothalamic gene expression was evaluated using a cDNA macroarray approach. mRNA and protein expressions were determined by reverse-transcription PCR (RT-PCR) and immunoblot. Cold exposure led to an increased expression of 43 genes and to a reduced expression of four genes. HF diet promoted an increased expression of 90 genes and a reduced expression of 78 genes. Only two genes (N-methyl-D-aspartate (NMDA) receptor 2B and guanosine triphosphate (GTP)-binding protein G-alpha-i1) were similarly affected by both thermogenesis-inducing conditions, undergoing an increment of expression. RT-PCR and immunoblot evaluations confirmed the modulation of NMDA receptor 2B and GTP-binding protein G-alpha-i1, only. This corresponds to 0.93% of all the responsive genes and 0.17% of the analyzed genes. These results indicate that distinct environmental thermogenic stimuli can modulate predominantly distinct profiles of genes reinforcing the complexity and multiplicity of the hypothalamic mechanisms that regulate energy conservation and expenditure.en
dc.relation.ispartofObesity
dc.publisher.countryEstados Unidospt
dc.publisherNATURE PUBLISHING GROUPpt
dc.date.issued2008pt
dc.identifier.citationOBESITY, v.16, n.6, p.1239-1247, 2008pt
dc.language.isoengpt
dc.description.volume16pt
dc.description.issuenumber6pt
dc.description.initialpage1239pt
dc.description.lastpage1247pt
dc.rightsfechadopt
dc.rights.holderCopyright NATURE PUBLISHING GROUPpt
dc.sourceWOSpt
unicamp.cruespUSPpt
dc.identifier.issn1930-7381pt
dc.identifier.doi10.1038/oby.2008.53pt
dc.identifier.urlhttp://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000256353700012&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecorden
dc.identifier.urlhttp://dx.doi.org/10.1038/oby.2008.53en
dc.date.available2013-07-26T17:58:47Z
dc.date.available2016-07-01T15:42:48Z-
dc.date.accessioned2013-07-26T17:58:47Z
dc.date.accessioned2016-07-01T15:42:48Z-
dc.description.provenanceMade available in DSpace on 2013-07-26T17:58:47Z (GMT). No. of bitstreams: 2 art_SOUZA_Distinct_subsets_of_hypothalamic_genes_are_modulated_2008.pdf.txt: 45771 bytes, checksum: deb823a87626ab82d792e34cefb29628 (MD5) art_SOUZA_Distinct_subsets_of_hypothalamic_genes_are_modulated_2008.pdf: 200365 bytes, checksum: 66aba4e398480a39a31a4ac1969e11e7 (MD5) Previous issue date: 2008en
dc.description.provenanceMade available in DSpace on 2016-07-01T15:42:48Z (GMT). No. of bitstreams: 2 art_SOUZA_Distinct_subsets_of_hypothalamic_genes_are_modulated_2008.pdf.txt: 45771 bytes, checksum: deb823a87626ab82d792e34cefb29628 (MD5) art_SOUZA_Distinct_subsets_of_hypothalamic_genes_are_modulated_2008.pdf: 200365 bytes, checksum: 66aba4e398480a39a31a4ac1969e11e7 (MD5) Previous issue date: 2008en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/920
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/920-
dc.contributor.departamentMedicina Interna
dc.contributor.unidadeFCMpt
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