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Type: Artigo de evento
Title: Enzymatic Hydrolyze Of Soy Oil Using Lipase By Geotrichum Candidum
Author: Maldonado R.R.
Fogaca F.M.
Ramos G.G.
Macedo G.A.
Rodrigues M.I.
Abstract: In recent years, lipase production by microorganisms has been extensively studied and different applications have been proposed for this enzyme. The aim of this work was to use the lipase generated by Geotrichum candidum to perform the soy oil hydrolysis as a first step of the enzymatic biodiesel production. The enzymatic hydrolyze was carried out through central composite design 2 3 under conditions of water (from 5 to 15% w/w), temperature (from 34 to 40X)) and enzyme concentration (from 3 to 5% w/w that means from 42 to 70 U EtL/g oil) in a thermostatic reactor using magnetic agitation. Lipase used codify as EtL was obtained using lipase solution with 15.0U/mL purification through ethanol addition and lyophilization for 2 days resulting in enzymatic solid containing 692 U/mg and 13.7% of water. All experiments was carried out with 2.Og of soybean oil and best hydrolysis result (34%) was obtained at 40°C, with 15% (m/m) of water and 3% of enzymatic solid (42 U/g oil), after 24 hours of reaction. In another experiment, using more oil (10g) and enzymatic solid with 925 U/g ,better results were obtained: 40%, 53% and 29% of hydrolysis after 12, 24 and 48 hours of reaction, respectively, with similar conditions: 15% (m/m) of water and 5% (m/m) of enzyme (46 U/g oil), at 37°C. These res ults demonstrate this lipase's good capability for the soy oil hydrolysis, and its related production of free fatty acids, indicating that it could represent an appropriate option for the biodiesel production.
Rights: fechado
Identifier DOI: 
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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