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Type: Artigo de periódico
Title: Inulinase Production By Agro-industrial Residues: Optimization Of Pretreatment Of Substrates And Production Medium
Author: Sguarezi C.
Longo C.
Ceni G.
Boni G.
Silva M.F.
DiLuccio M.
Mazutti M.A.
Maugeri F.
Rodrigues M.I.
Treichel H.
Abstract: In this work, production of inulinase was studied. Media formulation was optimized by experimental design and response surface techniques, as well as the pretreatment of the agro-industry residues used in the formulation of fermentation medium. Two agro-industry residues were investigated: sugarcane molasses (SCM) and corn steep liquor (CSL). Pretreatment with sulfuric acid was the most effective for clarification of SCM (pH 5.0, 24 h of resting time and final pH 4.0). Clarification of CSL was accomplished with phosphoric acid (pH 3.0, 24 h of resting time and final pH 5.5). A color reduction of approximately 70% was achieved for both substrates. The highest production of inulinase was obtained in a medium containing 100 g l -1 of pretreated SCM, 100 g l -1 of pretreated CSL and 6 g l -1 of Prodex Lac (yeast hydrolysate), yielding 1,139 U ml -1. © Springer Science + Business Media, LLC 2007.
Rights: fechado
Identifier DOI: 10.1007/s11947-007-0042-x
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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