Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/91702
Type: Artigo de periódico
Title: Cyclodextrin Glycosyltransferase Production By The Bacillus Sp., Subgroup Alcalophilus Using A Central Composite Design
Author: Blanco K.C.
de Lima C.J.B.
de Oliveira P.A.P.L.V.
Piao A.C.S.
Contiero J.
Abstract: Cyclodextrin glycosyltransferase (CGTase) activity was produced by the Bacillus sp., subgroup alcalophilus in a culture medium containing cassava starch. A central composite design and response surface methodology were used to study the influence of carbon source (cassava starch), nitrogen sources (yeast extract and tryptone) and sodium carbonate in the production medium. Assays were performed in 300 mL Erlenmeyer flasks containing 100 mL of production medium maintained in a shaker at 150 rpm at 35±1°C for 72 h of fermentation. The independent variables [0.75% cassava starch, nitrogen sources (0.375% yeast extract and 0.375% tryptone) and 1% Na2CO3] produced an enzyme activity of 96.07 U mL-1.© Academic Journals Inc.
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Rights: aberto
Identifier DOI: 10.3923/jm.2009.450.459
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-75849156578&partnerID=40&md5=c9d1bc9491aead4c2a1b3469ea5ab991
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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