Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/91650
Type: Artigo de periódico
Title: Crystallization And Preliminary X-ray Diffraction Analysis Of Q4dv70 From Trypanosoma Cruzi, A Hypothetical Protein With A Putative Thioredoxin Domain
Author: Dos Santos C.R.
Fessel M.R.
Vieira L.D.C.
Krieger M.A.
Goldenberg S.
Guimaraes B.G.
Zanchin N.I.T.
Barbosa J.A.R.G.
Abstract: Q4DV70 is annotated in the Trypanosoma cruzi CL Brener genome as a hypothetical protein with a predicted thioredoxin-like fold, although the catalytic cysteine residues that are conserved in typical oxidoreductases are replaced by serine residues. Gene-expression analysis indicates that this protein is differentially expressed during the T. cruzi life cycle, suggesting that it plays an important role during T. cruzi development. The gene coding for Q4DV70 was cloned and the protein was overexpressed in Escherichia coli with an N - terminal His tag. Purification of Q4DV70 was carried out by affinity and size-exclusion chromatography and the His tag was removed by TEV protease digestion. Crystals of Q4DV70 were grown using the sitting-drop vapour-diffusion method. A diffraction data set was collected to 1.50 Å resolution from a single crystal grown in 25% PEG 1500, 200 mM sodium thiocyanate pH 6.9, 10 mM phenol and 10% ethylene glycol. The crystal belonged to space group P212121, with unit-cell parameters a = 35.04, b = 50.32, c = 61.18 Å. The Q4DV70 structure was solved by molecular replacement using protein disulfide isomerase from yeast (PDB code 2b5e) as a search model. Initial refinement of the model indicated that the solution was correct. These data are being used for refinement of the model of Q4DV70. © 2009 International Union of Crystallography. All rights reserved.
Editor: 
Rights: aberto
Identifier DOI: 10.1107/S1744309109017734
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-67649227258&partnerID=40&md5=69a15ae44330059db8b5d270359ac7a3
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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