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|Type:||Capítulo de livro|
|Title:||Mperometric Biosensor Based On Laccase And Meldola's Blue Coimmobilized On Oxidized Multi-wall Carbon Nanotubes For Phenol Compounds Determination|
|Abstract:||In the present work is reported the development of an amperometric biosensor for phenol detection based in the coimmobilization of the Meldolas Blue, substituting the role of the molecular oxygen in the regeneration of the enzyme and laccase via EDC on oxidized multi-wall carbon nanotubes (MWCToxi). The variables that exert influence on the performance of the biosensor response, including enzyme immobilization procedure, laccase amounts, pH and working potential were investigated. Furthermore, the feasibility of the biosensor response for various phenol compounds was also investigated. The amperometric response for catechol using the proposed biosensor showed a wide linear response range (1 to 150 μmol L-1), good sensitivity (57.1 μA cm-2 μmol-1 L), excellent operational stability (after 200 determinations the response remained at 97 %) and very good storage stability (lifetime > 3 months). The results were compared with laccase immobilized on graphite powder, highlighting the remarkable features of MWCToxi in the biosensor performance. According to these features, it is possible to affirm that the developed biosensor is promising tool for phenol detection due to its good electrochemical response and enzyme stabilization. © 2010 Nova Science Publishers, Inc. All rights reserved.|
|Editor:||Nova Science Publishers, Inc.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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