Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/91320
Type: Artigo de periódico
Title: Synthesis Of Peptides From α- And β-tubulin Containing Glutamic Acid Side-chain Linked Oligo-glu With Defined Length
Author: Tegge W.
Bonafe C.F.S.
Teichmann A.
Erck C.
Abstract: Side-chain oligo- and polyglutamylation represents an important posttranslational modification in tubulin physiology. The particular number of glutamate units is related to specific regulatory functions. In this work, we present a method for the synthesis of building blocks for the Fmoc synthesis of peptides containing main chain glutamic acid residues that carry side-chain branching with oligo-glutamic acid. The two model peptide sequences CYEEVGVDSVEGEG-E(E x)-EEGEEY and CQDATADEQG-E(E x)-FEEEEGEDEA from the C-termini of mammalian α1- and β1-tubulin, respectively, containing oligo-glutamic acid side-chain branching with lengths of 1 to 5 amino acids were assembled in good yield and purity. The products may lead to the generation of specific antibodies which should be important tools for a more detailed investigation of polyglutamylation processes. © 2010 Werner Tegge et al.
Editor: 
Rights: aberto
Identifier DOI: 10.1155/2010/189396
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-84879192609&partnerID=40&md5=64cfeb2b0d40c3dbfe569cd0fbba15a8
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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