Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.typeArtigo de periódicopt_BR
dc.titleHypoxia, Hypoxia-inducible Factor-1α And Vascular Endothelial Growth Factor In A Murine Model Of Schistosoma Mansoni Infectionpt_BR
dc.contributor.authorAraujo A.P.pt_BR
dc.contributor.authorFrezza T.F.pt_BR
dc.contributor.authorAllegretti S.M.pt_BR
dc.contributor.authorGiorgio S.pt_BR
unicamp.authorAraújo, A.P., Department of Animal Biology, Biology Institute, Universidade Estadual de Campinas, Caixa Postal 6109, Cep 13083-970, Campinas, SP, Brazilpt_BR
unicamp.authorFrezza, T.F., Department of Animal Biology, Biology Institute, Universidade Estadual de Campinas, Caixa Postal 6109, Cep 13083-970, Campinas, SP, Brazilpt_BR
unicamp.authorAllegretti, S.M., Department of Animal Biology, Biology Institute, Universidade Estadual de Campinas, Caixa Postal 6109, Cep 13083-970, Campinas, SP, Brazilpt_BR
unicamp.authorGiorgio, S., Department of Animal Biology, Biology Institute, Universidade Estadual de Campinas, Caixa Postal 6109, Cep 13083-970, Campinas, SP, Brazilpt_BR
dc.description.abstractSchistosomiasis mansoni is a chronic parasitic disease where much of the symptomatology is attributed to granuloma formation, an immunopathological reaction against Schistosoma eggs. To more clearly understand the immunopathology of schistosomiasis, the tissue microenvironment generated by S. mansoni infected mice was investigated. Using the hypoxia marker pimonidazole, we provide immunohistochemical evidence that hypoxia occurred in inflammatory cells infiltrated around the eggs and cells surrounding granulomas in the liver, intestine, spleen and lungs of infected mice. Hypoxia-inducible factor-1α (HIF-1α) was mainly expressed in inflammatory cells surrounding the eggs and in hepatocytes surrounding cellular and fibrocellular granulomas in infected mouse liver. HIF-1α expression was also verified in granulomas in the other tissues tested (intestine, spleen and lungs). Vascular endothelial growth factor (VEGF) expression was observed in the extracellular space surrounding inflammatory cells in liver granuloma. The VEGF expression pattern verified in infected mouse liver was very similar to that observed in the other tissues tested. A strong positive correlation occurred between pimonidazole binding and HIF-1α and VEGF expression in the tissues tested, except for lung. This work is the first evidence that infection by a helminth parasite, S. mansoni, produces a hypoxic tissue microenvironment and induces HIF-1α and VEGF expression. © 2010 Elsevier Inc.en
dc.relation.ispartofExperimental and Molecular Pathologypt_BR
dc.identifier.citationExperimental And Molecular Pathology. , v. 89, n. 3, p. 327 - 333, 2010.pt_BR
dc.description.provenanceMade available in DSpace on 2015-06-26T12:35:44Z (GMT). No. of bitstreams: 1 2-s2.0-78249235607.pdf: 2832909 bytes, checksum: c6f7dc925f68a22bbe2922dc2350ee41 (MD5) Previous issue date: 2010en
dc.description.provenanceMade available in DSpace on 2015-11-26T15:25:48Z (GMT). No. of bitstreams: 2 2-s2.0-78249235607.pdf: 2832909 bytes, checksum: c6f7dc925f68a22bbe2922dc2350ee41 (MD5) 2-s2.0-78249235607.pdf.txt: 39171 bytes, checksum: 7a9f2c89a2357931d93894ae9bee0f65 (MD5) Previous issue date: 2010en
dc.description.referenceAlbina, J.E., Mastrofrancesco, B., Vessella, J.A., Louis, C.A., Henry, W.L., Reichner, J.S., HIF-1 expression in healing wounds: HIF-1alpha induction in primary inflammatory cells by TNF-alpha (2001) Am. J. Physiol. Cell Physiol., 281, pp. C1971-C1977pt_BR
dc.description.referenceAndrade, Z.A., Cheever, A.W., Alterations of the intrahepatic vasculature in hepatosplenic Schistosomiasis mansoni (1971) Am. J. Trop. Med. Hyg., 20, pp. 425-432pt_BR
dc.description.referenceArrais-Silva, W.W., Paffaro, V.A., Yamada, A.T., Giorgio, S., Expression of hypoxia-inducible factor-1α in the cutaneous lesions of BALB/c mice infected with Leishmania amazonensis (2005) Exp. Mol. Pathol., 78, pp. 49-54pt_BR
dc.description.referenceBaptista, A.P., Andrade, A.A., Angiogenesis and schistosomal granuloma formation (2005) Mem. Inst. Oswaldo Cruz, 100, pp. 183-185pt_BR
dc.description.referenceBassnett, S., McNulty, R., The effect of elevated intraocular oxygen on organelle degradation in the embryonic chicken lens (2003) J. Exp. Biol., 206, pp. 4353-4361pt_BR
dc.description.referenceBlouin, C.C., Page, E.L., Soucy, G.M., Richard, D.E., Hypoxic gene activation by lipopolysaccharide in macrophages: implication of hypoxia-inducible factor 1alpha (2004) Blood, 103, pp. 1124-1130pt_BR
dc.description.referenceBotusan, I.R., Sunkari, V.G., Savu, O., Catrina, A.I., Grünler, J., Lindberg, S., Pereira, T., Catrina, S.B., Stabilization of HIF-1alpha is critical to improve wound healing in diabetic mice (2008) Proc. Natl Acad. Sci., 105, pp. 19426-19431pt_BR
dc.description.referenceBurke, M.L., Jones, M.K., Gobert, G.N., Li, Y.S., Ellis, M.K., McManus, D.P., Immunopathogenesis of human schistosomiasis (2009) Parasite Immunol., 31, pp. 163-176pt_BR
dc.description.referenceCarmeliet, P., Angiogenesis in life, disease and medicine (2005) Nature, 438, pp. 932-936pt_BR
dc.description.referenceCatrina, S.B., Okamoto, K., Pereira, T., Brismar, K., Poellinger, L., Hyperglycemia regulates hypoxia-inducible factor-1alpha protein stability and function (2004) Diabetes, 53, pp. 3226-3232pt_BR
dc.description.referenceCheever, A.W., Lenzi, J.A., Lenzi, H.L., Andrade, Z.A., Experimental models of Schistosoma mansoni infection (2002) Mem. Inst. Oswaldo Cruz, 97, pp. 917-940pt_BR
dc.description.referenceCouto, J.L.A., Vieira, R., Barbosa, J.M., Machado, S.S., Ferreira, H.S., Alterações da função hepática de camundongos desnutridos e infectados pelo Schistosoma mansoni (2008) Rev. Soc. Bras. Med. Trop., 41, pp. 390-393pt_BR
dc.description.referenceDegrossoli, A., Bosetto, M.C., Lima, C.B.C., Giorgio, S., Expression of hypoxia-inducible factor 1α in mononuclear phagocytes infected with Leishmania amazonensis (2007) Immunol. Lett., 114, pp. 119-125pt_BR
dc.description.referenceEdungbola, L.C., Shciller, L.E., Histopathology of hepatic and pulmonary granulomata experimentally induced with eggs of Schistosoma mansoni (1979) J. Parasitol., 65, pp. 253-261pt_BR
dc.description.referenceFeldman, G.M., Dannenberg, A.M., Seed, J.L., Physiologic oxygen tensions limit oxidant-mediated killing of schistosome eggs by inflammatory cells and isolated granulomas (1990) J. Leukoc. Biol., 47, pp. 344-354pt_BR
dc.description.referenceFreedman, D.O., Ottesen, E.A., Eggs of Schistosoma mansoni stimulate endothelial cell proliferation in vitro (1988) J. Infect. Dis., 158, pp. 556-562pt_BR
dc.description.referenceGobert, G.M., Chai, M., McManus, D.P., Biology of the schistosome lung-stage schistosomulum (2007) Parasitology, 134, pp. 453-460pt_BR
dc.description.referenceHaeberle, H.A., Durrstein, C., Rosenberger, P., Hosakote, Y.M., Kuhlicke, J., Kempf, V.A., Garofalo, R.P., Eltzschig, H.K., Oxygen-independent stabilization of hypoxia inducible factor (HIF)-1 during RSV infection (2008) PLoS ONE, 3, pp. e3352pt_BR
dc.description.referenceHöckel, M., Vaupel, P., Tumor hypoxia: definitions and current clinical, biologic, and molecular aspects (2001) J. Natl Cancer Inst., 93, pp. 266-276pt_BR
dc.description.referenceJanssen, H.L., Haustermans, K.M., Sprong, D., Blommestijn, G., Hofland, I., Hoebers, F.J., Blijweert, E., Begg, C., HIF-1A, pimonidazole, and iododeoxyuridine to estimate hypoxia and perfusion in human head-and-neck tumors (2002) Int. J. Radiat. Oncol. Biol. Phys., 54, pp. 1537-1549pt_BR
dc.description.referenceJiang, B.H., Semenza, G.L., Bauer, C., Marti, H.H., Hypoxia-inducible factor 1 levels vary exponentially over a physiologically relevant range of O 2 tension (1996) Am. J. Physiol., 271, pp. C1172-C1180pt_BR
dc.description.referenceKanse, S.M., Liang, O., Schubert, U., Haas, H., Preissner, K.T., Doenhoff, M.J., Dennis, R.D., Characterization and partial purification of Schistosoma mansoni egg-derived pro-angiogenic factor (2005) Mol. Biochem. Parasitol., 144, pp. 76-85pt_BR
dc.description.referenceKempf, V.A.J., Lebiedziejewski, M., Alitalo, K., Wälzlein, J., Ehehalt, U., Fiebig, J., Huber, S., Autenrieth, I.B., Activation of hypoxia-inducible factor-1 in bacillary angiomatosis. Evidence for a role of hypoxia-inducible factor-1 in bacterial infections (2005) Circulation, 111, pp. 1054-1062pt_BR
dc.description.referenceKietzmann, T., Görlach, A., Reactive oxygen species in the control of hypoxia-inducible-factor-mediated gene expression (2005) Semin. Cell Dev. Biol., 16, pp. 474-486pt_BR
dc.description.referenceKlinkenberg, L.G., Sutherland, L.A., Bishai, W.R., Karakousis, P.C., Metronidazol lacks activity against Mycobacterium tuberculosis in an In Vivo hypoxic granuloma model of latency (2008) J. Infect. Dis., 198, pp. 275-283pt_BR
dc.description.referenceLenzi, H.L., Romanha, W.S., Santos, R.M., Rosas, A., Mota, E.M., Manso, P.P., Caputo, L.F., Pelajo-Machado, M., Four whole-istic aspects of schistosome granuloma biology: fractal arrangement, internal regulation, autopoietic component and closure (2006) Mem. Inst. Oswaldo Cruz, 101, pp. 219-231pt_BR
dc.description.referenceLoeffler, D.A., Lundy, S.K., Singh, K.P., Gerard, H.C., Hudson, A.P., Boros, D.L., Soluble egg antigens from Schistosoma mansoni induce angiogenesis-related processes by up-regulating vascular endothelial growth factor in human endothelial cells (2002) J. Infect. Dis., 185, pp. 1650-1656pt_BR
dc.description.referenceLopes, I.C., Santos, V.C.R., Souza, V.L.R.B., Rodrigues, I.R.C., Histopathological study of Schistosoma mansoni infection in the murine model using the PC (Pará) and LILA (Maranhão) strains (2006) Mem. Inst. Oswaldo Cruz, 10, pp. 273-277pt_BR
dc.description.referenceNizet, V., Johnson, R.S., Interdependence of hypoxic and innate immune responses (2009) Nat. Rev. Immunol., 9, pp. 609-617pt_BR
dc.description.referencePagé, E.L., Chan, D.A., Giaccia, A.J., Levine, M., Richard, D.E., Hypoxia-inducible factor-1alpha stabilization in nonhypoxic conditions: role of oxidation and intracellular ascorbate depletion (2008) Mol. Biol. Cell, 19, pp. 86-94pt_BR
dc.description.referencePark, J.E., Keller, G.A., Ferreira, N., The vascular endothelial growth factor (VEGF) isoforms: differential deposition into the subepithelial extracellular matrix and bioactivity of extracellular matrix-bound VEGF (1993) Mol. Biol. Cell, 4, pp. 1317-1326pt_BR
dc.description.referencePearce, E.J., MacDonald, A.S., The immunobiology of schistosomiasis (2002) Nat. Rev. Immunol., 2, pp. 499-511pt_BR
dc.description.referencePugh, C.W., Ratcliffe, P.J., Regulation of angiogenesis by hypoxia: role of the HIF system (2003) Nat. Med., 9, pp. 677-684pt_BR
dc.description.referenceRaleigh, J.A., Chou, S.C., Arteel, G.E., Horsman, M.R., Comparisons among pimonidazole binding, oxygen electrode measurements, and radiation response in C3H mouse tumors (1999) Radiat. Res., 151, pp. 580-589pt_BR
dc.description.referenceRamos, M.A., Kuzuya, M., Esaki, T., Miura, S., Satake, S., Asai, T., Kanda, S., Iguchi, A., Induction of macrophage VEGF in response to oxidized LDL and VEGF accumulation in human atherosclerotic lesions (1998) Arterioscler. Thromb. Vasc. Biol., 18, pp. 1188-1196pt_BR
dc.description.referenceRasey, J.S., Hofstrand, P.D., Chin, L.K., Tewson, T.J., Characterization of [18F]fluoroetanidazole, a new radiopharmaceutical for detecting tumor hypoxia (1999) J. Nucl. Med., 40, pp. 1072-1079pt_BR
dc.description.referenceSemenza, G.L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., Yu, A., Hypoxia, HIF-1, and the pathophysiology of common human disease (2000) Adv. Exp. Med. Biol., 475, pp. 123-130pt_BR
dc.description.referenceShi, S.R., Key, M.E., Kalra, K.L., Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections (1991) J. Histochem. Cytochem., 39, pp. 741-748pt_BR
dc.description.referenceSobhanifar, S., Aquino-Parsons, C., Stanbridge, E.J., Olive, P., Reduced expression of hypoxia-inducible factor-1alpha in perinecrotic regions of solid tumors (2005) Cancer Res., 65, pp. 7259-7266pt_BR
dc.description.referenceSpear, W., Chan, D., Coppens, I., Johnson, R.S., Giaccia, A., Blader, I.J., The host cell transcription factor hypoxia-inducible factor 1 is required for Toxoplasma gondii growth and survival at physiological oxygen levels (2006) Cell. Microbiol., 8, pp. 339-352pt_BR
dc.description.referenceStadecker, M.J., The development of granulomas in schistosomiasis: genetic backgrounds, regulatory pathways, and specific egg antigen responses that influence the magnitude of disease (1999) Microbes Infect., 1, pp. 505-510pt_BR
dc.description.referenceStavitsky, A.B., Regulation of granulomatous inflammation in experimental models of schistosomiasis (2004) Infect. Immun., 72, pp. 1-12pt_BR
dc.description.referenceUchida, T., Rossignol, F., Matthay, M.A., Mounier, R., Couette, S., Clottes, E., Clerici, C., Prolonged hypoxia differentially regulates hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha expression in lung epithelial cells: implication of natural antisense HIF-1alpha (2004) J. Biol. Chem., 279, pp. 14871-14878pt_BR
dc.description.referenceVaupel, P., Harrison, L., Tumor hypoxia: causative factors, compensatory mechanisms, and cellular response (2004) Oncologist, 9, pp. 4-9pt_BR
dc.description.referenceVia, L.E., Lin, P.L., Ray, S.M., Carrillo, J., Allen, S.S., Eum, S.I., Taylor, K., Barry, C.E., Tuberculous granulomas are hypoxic in guinea pigs, rabbits, and nonhuman primates (2008) Infect. Immun., 76, pp. 2333-2340pt_BR
dc.description.referencevon Lichtenberg, F., Sher, A., McIntyre, S., A lung model of schistosome immunity in mice (1977) Am. J. Pathol., 87, pp. 105-123pt_BR
dc.description.referenceVordermark, D., Kraft, P., Katzer, A., Bölling, T., Willner, J., Flentje, M., Glucose requirement for hypoxic accumulation of hypoxia-inducible factor-1alpha (HIF-1alpha) (2005) Cancer Lett., 30, pp. 122-133pt_BR
dc.description.referenceWilson, M.S., Mentink-Kane, M.M., Pesce, J.T., Ramalingam, T.R., Thompson, R., Wynn, T.A., Immunopathology of schistosomiasis (2007) Immunol. Cell Biol., 85, pp. 148-154pt_BR
dc.description.referenceYoshioka, L., Zanotti-Magalhães, E.M., Magalhães, L.A., Linares, A.X., Schistosoma ansoni: a study of pathogenesis of Santa Rosa strain (Campinas, SP, Brasil) in mice (2002) Rev. Soc. Bras. Med. Trop., 35, pp. 203-207pt_BR
dc.description.referenceZinkernagel, A.S., Johnson, R.S., Nizet, V., Hypoxia inducible factor (HIF) function in innate immunity and infection (2007) J. Mol. Med., 85, pp. 1339-1346pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
2-s2.0-78249235607.pdf2.77 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.