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Type: Artigo de periódico
Title: Mitochondria Generated Nitric Oxide Protects Against Permeability Transition Via Formation Of Membrane Protein S-nitrosothiols
Author: Leite A.C.R.
Oliveira H.C.F.
Utino F.L.
Garcia R.
Alberici L.C.
Fernandes M.P.
Castilho R.F.
Vercesi A.E.
Abstract: Mitochondria generated nitric oxide (NO) regulates several cell functions including energy metabolism, cell cycling, and cell death. Here we report that the NO synthase inhibitors (L-NAME, L-NNA and L-NMMA) administered either in vitro or in vivo induce Ca2+-dependent mitochondrial permeability transition (MPT) in rat liver mitochondria via a mechanism independent on changes in the energy state of the organelle. MPT was determined by the occurrence of cyclosporin A sensitive mitochondrial membrane potential disruption followed by mitochondrial swelling and Ca2+ release. In in vitro experiments, the effect of NOS inhibitors was dose-dependent (1 to 50μM). In addition to cyclosporin A, L-NAME-induced MPT was sensitive to Mg2+ plus ATP, EGTA, and to a lower degree, to catalase and dithiothreitol. In contrast to L-NAME, its isomer D-NAME did not induce MPT. L-NAME-induced MPT was associated with a significant decrease in both the rate of NO generation and the content of mitochondrial S-nitrosothiol. Acute and chronic in vivo treatment with L-NAME also promoted MPT and decreased the content of mitochondrial S-nitrosothiol. SNAP (a NO donor) prevented L-NAME mediated MPT and reversed the decrease in the rate of NO generation and in the content of S-nitrosothiol. We propose that S-nitrosylation of critical membrane protein thiols by NO protects against MPT. © 2010 Elsevier B.V.
Rights: fechado
Identifier DOI: 10.1016/j.bbabio.2010.01.034
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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