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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.identifier.isbnpt
dc.typeArtigo de periódicopt_BR
dc.titleIdentification Of Microcistin Lr At The Molecular Level Using Atomic Force Microscopy [identificação De Microcistina Lr Ao Nível Molecular Empregando Microscopia De Força Atômica]pt_BR
dc.contributor.authorEtchegaray A.pt_BR
dc.contributor.authorDe Castro Bueno C.pt_BR
dc.contributor.authorTeschke O.pt_BR
unicamp.authorTeschke, O., Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, CP 6165, 13083-970 Campinas - SP, Brazilpt_BR
unicamp.author.externalEtchegaray, A., Faculdade de Química, Pontifícia Universidade Católica de Campinas, CP 317, 13012-970 Campinas - SP, Brazilpt
unicamp.author.externalDe Castro Bueno, C., Faculdade de Química, Pontifícia Universidade Católica de Campinas, CP 317, 13012-970 Campinas - SP, Brazilpt
dc.description.abstractMicrocystins are non-ribosomal peptides that must be detected for its health concern. Here, microcystin LR and its specific antibody were respectively tethered to the substrate and to the tip of an atomic force microscope, after surface functionalization using 3-aminopropyltriethoxysilane and glutaraldehyde. Functionalization was confirmed comparing topographic images taken on bare and modified tips. Force versus distance curves were successfully used to measure the specific antibody-antigen interactions comparing with a control in which microcystin was initially blocked by incubation with free antibodies. The results showed unequivocally the specific recognition of MLR, suggesting that this method could be useful for biosensor development.en
dc.relation.ispartofQuimica Novapt_BR
dc.publisherpt_BR
dc.date.issued2010pt_BR
dc.identifier.citationQuimica Nova. , v. 33, n. 9, p. 1843 - 1848, 2010.pt_BR
dc.language.isoptpt_BR
dc.description.volume33pt_BR
dc.description.issuenumber9pt_BR
dc.description.initialpage1843pt_BR
dc.description.lastpage1848pt_BR
dc.rightsabertopt_BR
dc.sourceScopuspt_BR
dc.identifier.issn1004042pt_BR
dc.identifier.doi10.1590/S0100-40422010000900004pt_BR
dc.identifier.urlhttp://www.scopus.com/inward/record.url?eid=2-s2.0-79951662154&partnerID=40&md5=4d317db70aab61ef81cb49e51fdda0f6pt_BR
dc.date.available2015-06-26T12:34:34Z
dc.date.available2015-11-26T15:24:53Z-
dc.date.accessioned2015-06-26T12:34:34Z
dc.date.accessioned2015-11-26T15:24:53Z-
dc.description.provenanceMade available in DSpace on 2015-06-26T12:34:34Z (GMT). No. of bitstreams: 1 2-s2.0-79951662154.pdf: 5807393 bytes, checksum: 298568fb486f6480538f6138b42bd572 (MD5) Previous issue date: 2010en
dc.description.provenanceMade available in DSpace on 2015-11-26T15:24:53Z (GMT). No. of bitstreams: 2 2-s2.0-79951662154.pdf: 5807393 bytes, checksum: 298568fb486f6480538f6138b42bd572 (MD5) 2-s2.0-79951662154.pdf.txt: 36196 bytes, checksum: 7d615588f478b1eee4b903bf47c22219 (MD5) Previous issue date: 2010en
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/90822
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/90822-
dc.identifier.idScopus2-s2.0-79951662154pt_BR
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