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dc.typeArtigo de periódicopt_BR
dc.titleIdentification Of Microcistin Lr At The Molecular Level Using Atomic Force Microscopy [identificação De Microcistina Lr Ao Nível Molecular Empregando Microscopia De Força Atômica]pt_BR
dc.contributor.authorEtchegaray A.pt_BR
dc.contributor.authorDe Castro Bueno C.pt_BR
dc.contributor.authorTeschke O.pt_BR
unicamp.authorTeschke, O., Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, CP 6165, 13083-970 Campinas - SP, Brazilpt_BR, A., Faculdade de Química, Pontifícia Universidade Católica de Campinas, CP 317, 13012-970 Campinas - SP, Brazilpt Castro Bueno, C., Faculdade de Química, Pontifícia Universidade Católica de Campinas, CP 317, 13012-970 Campinas - SP, Brazilpt
dc.description.abstractMicrocystins are non-ribosomal peptides that must be detected for its health concern. Here, microcystin LR and its specific antibody were respectively tethered to the substrate and to the tip of an atomic force microscope, after surface functionalization using 3-aminopropyltriethoxysilane and glutaraldehyde. Functionalization was confirmed comparing topographic images taken on bare and modified tips. Force versus distance curves were successfully used to measure the specific antibody-antigen interactions comparing with a control in which microcystin was initially blocked by incubation with free antibodies. The results showed unequivocally the specific recognition of MLR, suggesting that this method could be useful for biosensor development.en
dc.relation.ispartofQuimica Novapt_BR
dc.identifier.citationQuimica Nova. , v. 33, n. 9, p. 1843 - 1848, 2010.pt_BR
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dc.description.provenanceMade available in DSpace on 2015-11-26T15:24:53Z (GMT). No. of bitstreams: 2 2-s2.0-79951662154.pdf: 5807393 bytes, checksum: 298568fb486f6480538f6138b42bd572 (MD5) 2-s2.0-79951662154.pdf.txt: 36196 bytes, checksum: 7d615588f478b1eee4b903bf47c22219 (MD5) Previous issue date: 2010en
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