Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Morphological, Biological And Molecular Characterization Of Three Strains Of Trypanosoma Cruzi Chagas, 1909 (kinetoplastida, Trypanosomatidae) Isolated From Triatoma Sordida (stal) 1859 (hemiptera, Reduviidae) And A Domestic Cat
Author: Rimoldi A.
Toma Alves R.
Ambra sio D.L.
Fernandes M.Z.T.
Martinez I.
De Araujo R.F.
Cicarelli R.M.B.
Da Rosa J.A.
Abstract: A study was conducted of the biological, morphological and molecular characters of 3 strains of Trypanosoma cruzi (SI5, SI8 and SIGR3) isolated from specimens of Triatoma sordida collected in Santo Inãcio and a domestic cat. In order to carry out the study, the following parameters were evaluated: pre-patent period, parasitaemia curves, morphology of the parasites, mortality rates, histopathological lesions and molecular typing. The strains presented variable pre-patent periods, low parasitaemia and no animal mortality. The morphological study of trypomastigotes showed a predominance of intermediate-width and short-length forms, as well as low nuclear index. Epimastigotes presented a low nuclear index, intermediate-width forms in strains SI5 and SI8, and large-width forms in SIGR3. A shorter length could be noted in strains SI8 and SIGR3, whereas SI5 displayed an intermediate length. The histopathological study did not detect amastigote nests in tissues. The amplification of the divergent domain of 24S rRNA, HSP60 and GPI genes of strains SI5, SI8 and SIGR3 classified the 3 strains into Group II. Biological parameters made it possible to classify the strains isolated in Santo Inãcio (BA) into Biodeme III, Zymodeme 1 and Group II of T. cruzi. © Cambridge University Press 2011.
Rights: fechado
Identifier DOI: 10.1017/S0031182011001697
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.