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|Type:||Artigo de periódico|
|Title:||Cd63 And Cd123 Expression, Igg Autoantibody And Accuracy Of Autologous Serum Test In Patients With Chronic Urticaria [cd63 E Cd123 Expressão, Autoanticorpos Igg E Acurácia Do Teste Do Soro Autólogo Em Pacientes Com Urticária Crônica]|
Da Silveira Antunes R.N.
De Almeida Filho O.M.
Baleotti Jr. W.
De Aguiar Cavaretto D.
|Abstract:||Introduction: The autologous serum skin test (ASST) may suggest an autoimmune etiology in chronic urticaria (CU). A new laboratory technique called basophil activation test (BAT) has been currently employed for its diagnosis. Objective: To analyze ASST in relation to BAT as well as to evaluate interleukin 3 (IL3) receptors (CD123) and non-specific immunoglobulin G (IgG) autoantibodies bound to basophils in patients with chronic urticaria. Methods: We studied 33 adults with CU and mean age of 42.5 + 14 years. After stimulation by serum from patients with CU, CD63 expression on basophils from one atopic donor was analyzed by flow cytometry. Furthermore, we investigated CD123 and IgG autoantibody expressions. Results: The odds ratio (OR) between ASST and BAT was 1.00 (95% confidence interval [CI]: 0.22 to 4.5). The ASST for autoimmune CU diagnosis showed an accuracy of 54.5%, sensitivity of 66%, specificity of 33%, positive predictive value of 63%, and negative predictive value of 36%. There was no statistical difference between the studied groups as to mean non-specific IgG and CD123 expressions (for a p < 0.05). Discussion: This study demonstrated that ASST has low accuracy in the diagnosis of autoimmune CU. Concerning other analyzed aspects, there was no statistical difference between positive ASST and negative ASST. Conclusions: Due to insufficient studies in this area and the relevance of this issue, further investigation is required.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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