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|Type:||Artigo de periódico|
|Title:||The Influence Of Cell Dimensions On The Vulnerability Of Ventricular Myocytes To Lethal Injury By High-intensity Electrical Fields [influência Das Dimensões Celulares Sobre A Vulnerabilidade De Miócitos Ventriculares Ao Efeito Letal De Campos Elétricos De Alta Intensidade]|
de Oliveira P.X.
|Abstract:||Application of high intensity electric fields (HIEF) to the myocardium is commonly used for cardiac defibrillation/cardioversion. Although effective at reversing life-threatening arrhythmias, HIEF may cause myocyte damage due to membrane electropermeabilization. In this study, the influence of cell length and width on HIEF-induced lethal injury was analyzed in isolated rat cardiomyocytes in parallel alignment with the field. The field-induced maximum variation of membrane potential (ΔVmax) was estimated with the Klee-Plonsey model. The studied myocyte population was arranged in two group pairs for comparison: the longest vs. the shortest cells, and the widest vs. narrowest cells. Threshold field intensity was significantly lower in the longest vs. shortest myocytes, whereas cell width influence was not significant. The threshold ΔVmax was comparable in all groups. Likewise, a significant leftward shift of the lethality curve (i.e., relationship of the probability of lethality vs. field intensity) of the longest cells was observed, evidencing greater sensitivity to HIEF-induced damage. However, the lethality curve as a function of ΔVmax was similar in all groups, confirming a prediction of the Klee-Plonsey model. The similar results for excitation and injury at threshold and HIEF stimulation, respectively, indicate that: a) the effect of cell length on the sensitivity to the field would be attributable to differences in field-induced membrane polarization that lead to excitation or lethal electroporation; b) the Klee-Plonsey model seems to be reliable for analysis of cell interaction with HIEF; c)it is possible that increased cell length in hypertrophied hearts enhances myocyte fragility upon defibrillation/cardioversion.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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