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Type: Artigo de periódico
Title: Arhgap21 Protein, A New Partner Of α-tubulin Involved In Cell-cell Adhesion Formation And Essential For Epithelial-mesenchymal Transition
Author: Barcellos K.S.A.
Bigarella C.L.
Wagner M.V.
Vieira K.P.
Lazarini M.
Langford P.R.
MacHado-Neto J.A.
Call S.G.
Staley D.M.
Chung J.Y.
Hansen M.D.
Saad S.T.O.
Abstract: Cell-cell adhesions and the cytoskeletons play important and coordinated roles in cell biology, including cell differentiation, development, and migration. Adhesion and cytoskeletal dynamics are regulated by Rho-GTPases. ARHGAP21 is a negative regulator of Rho-GTPases, particularly Cdc42. Here we assess the function ofARHGAP21in cell-cell adhesion, cell migration, and scattering. We find that ARHGAP21 is localized in the nucleus, cytoplasm, or perinuclear region but is transiently redistributed to cell-cell junctions 4 h after initiation of cell-cell adhesion. ARHGAP21 interacts with Cdc42, and decreased Cdc42 activity coincides with the appearance of ARHGAP21 at the cell-cell junctions. Cells lacking ARHGAP21 expression show weaker cell-cell adhesions, increased cell migration, and a diminished ability to undergo hepatocyte growth factor-induced epithelialmesenchymal transition (EMT). In addition, ARHGAP21 interacts with α-tubulin, and it is essential for α-tubulin acetylation in EMT. Our findings indicate that ARHGAP21 is a Rho-GAP involved in cell-cell junction remodeling and that ARHGAP21 affects migration and EMT through α-tubulin interaction and acetylation. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
Rights: fechado
Identifier DOI: 10.1074/jbc.M112.432716
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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