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Type: Artigo de periódico
Title: Identification Of Nuclear Factor-κb Sites In The Slc2a4 Gene Promoter
Author: Furuya D.T.
Neri E.A.
Poletto A.C.
Anhe G.F.
Freitas H.S.
Campello R.S.
Reboucas N.A.
Machado U.F.
Abstract: Glucose transporter GLUT4 protein, codified by Slc2a4 gene plays a key role in glycemic homeostasis. Insulin resistance, as in obesity, has been associated to inflammatory state, in which decreased GLUT4 is a feature. Inflammatory NF-κB transcriptional factor has been proposed as a repressor of Slc2a4; although, the binding site(s) in Slc2a4 promoter and the direct repressor effect have never been reported yet. A motif-based sequence analysis of mouse Slc2a4 promoter revealed two putative κB sites located inside -83/-62 and -134/-113. bp. Eletrophoretic mobility assay showed that p50 and p65 NF-κB subunits bind to both putative κB sites. Chromatin immunoprecipitation assay using genomic DNA from adipocytes confirmed p50- and p65-binding to Slc2a4 promoter. Moreover, transfection experiments revealed that NF-κB binds to the -134/-113. bp region of the mouse Slc2a4 gene promoter, inhibiting the Slc2a4 gene transcription. The current findings demonstrate the existence of two κB sites in Slc2a4 gene promote, and that NF-κB has a direct repressor effect upon the Slc2a4 gene, providing an important link between insulin resistance and inflammation. © 2013 Elsevier Ireland Ltd.
Rights: fechado
Identifier DOI: 10.1016/j.mce.2013.01.019
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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