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Type: Capítulo de livro
Title: Determination Of Phenolic Compounds Based On The Development Of Biosensors Using Nano-estructured Carbon Materials
Author: Rocha M.B.
Somera B.F.
Tarley C.R.T.
Clausen D.N.
Pereira A.C.
Abstract: The enzymatic biosensors present some unique characteristics as low cost, simplicity, selectivity and sensibility, which might turn them attractive for the determination of target compounds in complex matrix, for instance, the environmental ones. The objective of this work is to develop amperometric biosensors for the determination of the concentration of phenolic compounds in environmental samples using carbonaceous materials. It was studied three samples of modified xerogels, two with melamine (FRM2B-PN, HFRM2-PN), one with urea (HFRU2-PN 1/2), besides standard xerogel without modification (Xerogel pH 5.5), carbon nanotubes and graphite. It was also studied the enzymatic activity of the zucchini extract and it was characterized in the prepared paste with the mentioned materials. For the preparation of the electrochemical biosensors, some parameters such as enzymatic extract concentration, hydrogen peroxide concentration, pH and buffer solution were optimized. The higher enzymatic activity was obtained when the enzymatic extract was immobilized onto carbon nanotube. The peroxidase enzyme extracted from several zucchinis (natural source) presented lower activity value than those described in literature and also presented low stability. The amperometric biosensor optimization (work electrode prepared with carbon nanotube paste and enzymatic extract) was evaluated through cyclic voltammetry and it is possible to observe a higher sensibility to the prepared biosensor with 100 μL of enzymatic extract, when the electrochemical measures were made in buffer solution phosphate pH 6.5, containing 10 μmolL-1 of hydrogen peroxide. The activity of the enzymatic extract from the zucchini (natural source) presented low activity, but this extract can still be used in the immobilization of different employed materials. The carbon nanotubes, among the analyzed materials, show the best support for the enzyme immobilization. For the optimization of the biosensor, the result with best sensibility was verified in the electrode prepared with carbon nanotubes paste containing 100 μL of enzymatic extract, when used in a buffer solution phosphate pH 6.5, containing 10 μmolL-1 of hydrogen peroxide. © 2013 Nova Science Publishers, Inc. All rights reserved.
Editor: Nova Science Publishers, Inc.
Rights: fechado
Identifier DOI: 
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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