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Type: Artigo de periódico
Title: Autophagy And Proinfl Ammatory Cytokine Expression In The Intestinal Mucosa And Mesenteric Fat Tissue Of Patients With Crohn's Disease [autofagia E Expressão De Citocinas Pró-infl Amatórias Na Mucosa Intestinal E No Tecido Mesenterial De Pacientes Com Doença De Crohn]
Author: Leal R.F.
Milanski M.
Coy C.S.R.
Portovedo M.
Rodrigues V.S.
Coope A.
Ayrizono M.L.S.
Fagundes J.J.
Velloso L.A.
Abstract: Background: Recently, mesenteric fat has been proposed to play a role in the pathophysiology of Crohn's disease (CD), as fat hypertrophy is detected close to the affected intestinal area; however, there are few studies regarding autophagy and creeping fat tissue in CD. Objective: Evaluate autophagy-related proteins and proinfl ammatory cytokines in intestinal mucosa and mesenteric fat in patients with CD and controls. Patients and methods: Ten patients with CD, eight with non-infl ammatory disease who underwent surgery, and eight with normal ileocolonoscopy were studied. The expression of LC3-II, TNF-α and IL-23 was determined by immunoblot of protein extracts. In addition, total RNA of LC3 and Atg16-L1 were determined using RT-PCR. Results: The expression of LC3-II was signifi cantly lower in the mesenteric tissue of CD when compared to controls (p < 0.05). In contrast, the intestinal mucosa of the CD group had higher levels of LC3-II (p < 0.05). However, mRNA expression of autophagy-related proteins was similar when compared to mesenteric fat groups. TNF-α and IL-23 expressions were higher in intestinal mucosa of CD than in control (p < 0.05). Conclusion: These fi ndings suggest a defect in the autophagic activity of the creeping fat tissue in CD, which could be involved with the maintenance of the infl ammatory process in the intestinal mucosa. © 2013 Elsevier Editora Ltda. All rights reserved.
Rights: aberto
Identifier DOI: 
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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