Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/88171
Type: Artigo de periódico
Title: Increased Age Is Associated With Epigenetic And Structural Changes In Chromatin From Neuronal Nuclei
Author: Rodrigues H.F.
Souza T.A.
Ghiraldini F.G.
Mello M.L.S.
Moraes A.S.
Abstract: Chromatin organization has been considered to play a major role on aging, by regulating DNA accessibility to transcription and repair machinery. Such organization can be modulated by epigenetic events, such as DNA methylation and histone post-translational modifications. Since changes on gene expression profiles have been described in aged neurons, our aim was to study the age-dependent relationship between structural and epigenetic alterations on chromatin of cortical neurons from mice. For this purpose, isolated neuronal nuclei from mice of two ages were studied by image analysis after cytochemistry, or assessed for chromatin accessibility by enzymatic digestion. Additionally, two epigenetic marks, for open and for densely packed chromatin fibers were quantified. Results indicate epigenetically driven alterations on chromatin organization of cortical neurons with advancing age, whose fibers seem to undergo redistribution and unpackaging. Since increased transcriptional activity is not characteristic of aged neurons, these loosened chromatin fibers may be associated with impaired genome stability, as well as with increased accessibility of repair machinery to a life span damaged DNA. J. Cell. Biochem. 115: 659-665, 2014. © 2013 Wiley Periodicals, Inc. © 2013 Wiley Periodicals, Inc.
Editor: 
Rights: fechado
Identifier DOI: 10.1002/jcb.24705
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-84892908913&partnerID=40&md5=149cd7788961cf64230ad3f12da578ed
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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