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Type: Artigo de periódico
Title: Phytotoxicity And Identification Of Secondary Metabolites Of Sapindus Saponaria L. Leaf Extract
Author: Grisi P.U.
Forim M.R.
Costa E.S.
Anese S.
Franco M.F.
Eberlin M.N.
Gualtieri S.C.J.
Abstract: The aim of this study was to evaluate and quantify the phytotoxic effects of hexane and ethyl acetate fractions obtained from a leaf extract of Sapindus saponaria on the germination and seedling growth of Euphorbia heterophylla (wild poinsettia) and Echinochloa crus-galli (barnyardgrass) and to isolate and identify the major bioactive compounds. A crude ethanol extract was prepared from 100 g of dry plant material in 500 mL of ethanol. The extract was fractionated by liquid–liquid extraction, and the hexane and ethyl acetate fractions were solubilized at concentrations of 0.625, 1.25, 2.5, and 5.0 mg mL−1. The effect of these fractions was compared with the oxyfluorfen herbicide in bioassays. The hexane and ethyl acetate fractions inhibited germination, induced abnormalities, and reduced seedling growth of E. crus-galli and E. heterophylla with concentration-dependent effects. The phytotoxicity of the fractions ranged according to the receptor species, and the ethyl acetate fraction showed a greater inhibitory effect than the hexane fraction on seedling development. For both species, the oxyfluorfen herbicide inhibited mainly shoot growth, whereas the plant extracts inhibited seedling root growth. The S. saponaria fractions caused a reduction of more than 50 % in the size of metaxylem cells of E. heterophylla roots. The ethyl acetate fraction obtained from S. saponaria leaves was subjected to fractionation, and the substance isolated was identified as 3-(1,2-dimethyl-5-oxabicyclo [2.1.1] hexan-2-yl) but-2-enoic acid. This compound also inhibited the germination and seedling growth of E. crus-galli and E. heterophylla, but the ethyl acetate fraction was more potent in reducing seedling growth.
Editor: Springer New York LLC
Rights: fechado
Identifier DOI: 10.1007/s00344-014-9469-2
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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