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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampChiarotto, Gabriela Bortolançapt_BR
dc.contributor.authorunicampCavarretto, Gabrielapt_BR
dc.contributor.authorunicampBombeiro, André Luispt_BR
dc.contributor.authorunicampOliveira, Alexandre Leite Rodrigues dept_BR
dc.typeArtigopt_BR
dc.titleNeuroprotective effect of tempol (4 hydroxy-tempo) on neuronal death induced by sciatic nerve transection in neonatal ratspt_BR
dc.contributor.authorChiarotto, Gabriela Bortolancapt_BR
dc.contributor.authorDrummond, Luisapt_BR
dc.contributor.authorCavarretto, Gabrielapt_BR
dc.contributor.authorBombeiro, André Luispt_BR
dc.contributor.authorOliveira, Alexandre Leite Rodrigues dept_BR
unicamp.authorChiarotto, G.B., Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), CP 6109, CEP 13083-907 Campinas, SP, Brazilpt_BR
unicamp.authorDrummond, L., Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), CP 6109, CEP 13083-907 Campinas, SP, Brazilpt_BR
unicamp.authorCavarretto, G., Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), CP 6109, CEP 13083-907 Campinas, SP, Brazilpt_BR
unicamp.authorBombeiro, A.L., Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), CP 6109, CEP 13083-907 Campinas, SP, Brazilpt_BR
unicamp.authorDe Oliveira, A.L.R., Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), CP 6109, CEP 13083-907 Campinas, SP, Brazilpt_BR
dc.subjectNeuroproteçãopt_BR
dc.subjectNeurônios motorespt_BR
dc.subjectTempolpt_BR
dc.subjectMedula espinhalpt_BR
dc.subjectNervo isquiáticopt_BR
dc.subjectApoptosept_BR
dc.subject.otherlanguageNeuroprotectionpt_BR
dc.subject.otherlanguageMotor neuronspt_BR
dc.subject.otherlanguageTempolpt_BR
dc.subject.otherlanguageSpinal cordpt_BR
dc.subject.otherlanguageSciatic nervept_BR
dc.subject.otherlanguageApoptosispt_BR
dc.description.abstractPeripheral nerve injury in newborn rats triggers extensive neuronal death within the spinal cord. Because most neurodegeneration is related to oxidative stress and apoptosis, the use of antioxidants may be of therapeutic interest. Tempol is promising because of its ability to chelate reactive oxygen species and to minimize or even prevent tissue damage. Here, we evaluated neuroprotective effects of tempol following neonatal sciatic nerve transection. Two-day-old pups underwent sciatic nerve axotomy followed by tempol (12, 24 and 48. mg/kg) treatment (i.p.) at 10. min, 6. h, and every 24. h up to 1 week after injury. The rats were then killed for lumbar intumescence analysis. Nissl staining, TUNEL, synaptophysin immunolabeling and qRT-PCR (Caspase 3, Bax and Bcl2) were carried out. The results indicated that tempol treatment, at 24. mg/kg, increased up to 21% spinal cord motoneuron survival (p< 0.001), also preserving pre-synaptic terminals in the neuropile. Likewise, the TUNEL-positive cell number decreased in tempol-treated animals. qRT-PCR results indicated differential increase in Caspase 3 (3-fold), Bax (13-fold) and Bcl2 (28-fold) gene expression, after 12. h following axotomy and tempol treatment. In conclusion, tempol administration has proven to be neuroprotective after neonatal nerve injury, leading to improved motoneuron survival, synapse preservation and minimizing apoptosis. © 2014 Elsevier Inc.en
dc.description.abstractPeripheral nerve injury in newborn rats triggers extensive neuronal death within the spinal cord. Because most neurodegeneration is related to oxidative stress and apoptosis, the use of antioxidants may be of therapeutic interest. Tempol is promising becapt_BR
dc.relation.ispartofBrain research bulletinpt_BR
dc.relation.ispartofabbreviationBrain res. bull.pt_BR
dc.publisher.cityPhiladelphia, PApt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2014pt_BR
dc.date.monthofcirculationJulypt_BR
dc.identifier.citationBrain Research Bulletin. Elsevier Inc., v. 106, n. , p. 1 - 8, 2014.pt_BR
dc.language.isoengpt_BR
dc.description.volume106pt_BR
dc.description.firstpage1pt_BR
dc.description.lastpage8pt_BR
dc.rightsfechadopt_BR
dc.rightsfechadopt_br
dc.sourceSCOPUSpt_BR
dc.identifier.issn0361-9230pt_BR
dc.identifier.eissn1873-2747pt_BR
dc.identifier.doi10.1016/j.brainresbull.2014.04.010pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0361923014000641pt_BR
dc.description.sponsorshipFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorshipCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorship1FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorship1CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsordocumentnumber2011/12857-8pt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.date.available2015-06-25T18:03:51Z
dc.date.available2015-11-26T15:06:07Z-
dc.date.accessioned2015-06-25T18:03:51Z
dc.date.accessioned2015-11-26T15:06:07Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T15:06:07Z (GMT). No. of bitstreams: 2 2-s2.0-84899960608.pdf: 1847389 bytes, checksum: da9ea57d06044b54234296e2d9f5f54a (MD5) 2-s2.0-84899960608.pdf.txt: 34137 bytes, checksum: d5773e95f30f4cb2368838d3dbe827ad (MD5) Previous issue date: 2014en
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/88074
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/88074-
dc.identifier.idScopus2-s2.0-84899960608pt_BR
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dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordNeuronal survivalpt_BR
dc.identifier.source2-s2.0-84899960608pt_BR
dc.creator.orcid0000-0003-4177-8735pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0003-2303-8127pt_BR
dc.creator.orcid0000-0003-4224-4575pt_BR
dc.type.formArtigo de pesquisapt_BR
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