Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/87899
Type: Artigo de periódico
Title: Acute Exercise Decreases Trb3 Protein Levels In The Hypothalamus Of Obese Rats
Author: de Almeira Rodrigues B.
Pauli L.S.S.
de Souza C.T.
da Silva A.S.R.
Cintra D.E.C.
Marinho R.
de Moura L.P.
Ropelle E.C.C.
Botezelli J.D.
Ropelle E.R.
Pauli J.R.
Abstract: PURPOSE: To evaluate the effects of acute exercise on the TRB3 protein levels and interaction between TRB3/Akt proteins in the hypothalamus of obese rats. In addition, we evaluated the relationship between TRB3 and endoplasmic reticulum stress (ER stress) and verified whether an acute exercise session is able to influence these processes.METHODS: In the first part of the study, the rats were divided into three groups: control (lean) - fed with a standard rodent chow, DIO - fed with a high fat diet and DIO submitted to a swimming acute exercise protocol (DIO-EXE). In the second part of the study, we used other three groups: control (lean) receiving an intracerebroventricular (i.c.v.) infusion of vehicle, lean receiving an i.c.v. infusion of thapsigargin, and lean receiving an i.c.v infusion of thapsigargin and performing an acute exercise session. Four hours after the exercise session, the food intake was measured and the hypothalamus was dissected and separated for subsequent protein analysis by immunoblotting and Real Time PCR.RESULTS: The acute exercise session reduced the TRB3 protein levels, disrupted the interaction between TRB3/Akt proteins, increased the phosphorylation of Foxo1 and restored the anorexigenic effects of insulin in the hypothalamus of DIO rats. Interestingly, the suppressive effects of acute exercise on TRB3 protein levels may be related, at least in part, to the decrease of ER stress (evaluated though pancreatic ER kinase phosphorylation - pPERK and C/EBP homologous protein - CHOP protein levels) in the hypothalamus.CONCLUSION: In conclusion, the reduction of hypothalamic TRB3 protein levels mediated by exercise may be associated with the reduction of ER stress. These data provided a new mechanism by which an acute exercise session improves insulin sensitivity in hypothalamus and restores food intake control in obesity.
Editor: Lippincott Williams and Wilkins
Rights: fechado
Identifier DOI: 10.1249/MSS.0000000000000585
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-84912056757&partnerID=40&md5=b9187a574ff0f8247527142943653624
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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