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Type: Artigo de periódico
Title: A Putative Otu Domain-containing Protein 1 Deubiquitinating Enzyme Is Differentially Expressed In Thyroid Cancer And Identifies Less-aggressive Tumours
Author: Carneiro A.P.
Reis C.F.
Morari E.C.
Maia Y.C.P.
Nascimento R.
Bonatto J.M.C.
De Souza M.A.
Goulart L.R.
Ward L.S.
Abstract: Background: This study aimed to identify novel biomarkers for thyroid carcinoma diagnosis and prognosis.Methods:We have constructed a human single-chain variable fragment (scFv) antibody library that was selected against tumour thyroid cells using the BRASIL method (biopanning and rapid analysis of selective interactive ligands) and phage display technology.Results:One highly reactive clone, scFv-C1, with specific binding to papillary thyroid tumour proteins was confirmed by ELISA, which was further tested against a tissue microarray that comprised of 229 thyroid tissues, including: 110 carcinomas (38 papillary thyroid carcinomas (PTCs), 42 follicular carcinomas, 30 follicular variants of PTC), 18 normal thyroid tissues, 49 nodular goitres (NG) and 52 follicular adenomas. The scFv-C1 was able to distinguish carcinomas from benign lesions (P=0.0001) and reacted preferentially against T1 and T2 tumour stages (P=0.0108). We have further identified an OTU domain-containing protein 1, DUBA-7 deubiquitinating enzyme as the scFv-binding antigen using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry.Conclusions:The strategy of screening and identifying a cell-surface-binding antibody against thyroid tissues was highly effective and resulted in a useful biomarker that recognises malignancy among thyroid nodules and may help identify lower-risk cases that can benefit from less-aggressive management. © 2014 Cancer Research UK. All rights reserved.
Editor: Nature Publishing Group
Rights: fechado
Identifier DOI: 10.1038/bjc.2014.331
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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