Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/87552
Type: Artigo
Title: Identification of coagulase-negative staphylococci from bovine intramammary infection by matrix-assisted laser desorption ionization–time of flight mass spectrometry
Author: Tomazi, Tiago
Gonçalves, Juliano Leonel
Barreiro, Juliana Regina
Braga, Patrícia Aparecida de Campos
Prada e Silva, Luis Felipe
Eberlin, Marcos Nogueira
Santos, Marcos Veiga dos
Abstract: Coagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of the limitations related to the specific diagnosis of CoNS is the lack of an accurate, rapid, and convenient method that can differentiate the bacterial species comprising this group. The aim of this study was to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to accurately identify CoNS species in dairy cow IMI. In addition, the study aimed to determine the frequency of CoNS species causing bovine IMI. A total of 108 bacterial isolates were diagnosed as CoNS by microbiological cultures from two milk samples collected from 21 dairy herds; the first sample was collected at the cow level (i.e., 1,242 composite samples from all quarters), while the second sample was collected at the mammary quarter level (i.e., 1,140 mammary samples collected from 285 cows). After CoNS isolation was confirmed by microbiological culture for both samples, all CoNS isolates (n=108) were genotypically differentiated by PCR restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and subjected to the MALDI-TOF MS identification procedure. MALDI-TOF MS correctly identified 103 (95.4%) of the CoNS isolates identified by PCR-RFLP at the species level. Eleven CoNS species isolated from bovine IMI were identified by PCR-RFLP, and the most prevalent species was Staphylococcus chromogenes (n=80; 74.1%). In conclusion, MALDI-TOF MS may be a reliable alternative method for differentiating CoNS species causing bovine IMI. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Coagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of the limitations related to the specific diagnosis of CoNS is the lack of an accurate, rapid, and convenient
Subject: Estafilococos coagulase negativa
Mastite
Espectrometria de massas por ionização e dessorção a laser assistida por matriz
Country: Estados Unidos
Editor: American Society for Microbiology
Citation: Journal Of Clinical Microbiology. American Society For Microbiology, v. 52, n. 5, p. 1658 - 1663, 2014.
Rights: aberto
Aberto
Identifier DOI: 10.1128/JCM.03032-13
Address: https://jcm.asm.org/content/52/5/1658
Date Issue: 2014
Appears in Collections:IQ - Artigos e Outros Documentos

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