Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/87123
Type: Artigo de periódico
Title: Tnfα Contributes For Attenuating Both Y397fak And Y416src Phosphorylations In Osteoblasts
Author: Cavagis A.D.M.
Takamori E.R.
Granjeiro J.M.
Oliveira R.C.
Ferreira C.V.
Peppelenbosch M.P.
Zambuzzi W.F.
Abstract: Objective: Our poor understanding of how inflammatory mediators can affect osteoblast behavior led us to investigate the tumor necrosis factor (TNF)α-induced focal adhesion kinase (FAK) and Src phosphorylation. Material and Methods: MC3T3-E1 pre-osteoblast cells were harvested at specific time points after either TNFα treatment or RAW267 stimulated conditioned medium, and thereafter cell extracts were prepared for Immunoblotting assay. ELISA detected TNFα content at conditioned medium. Tumor necrosis factor-α-neutralizing antibodies also were used. Results: It was possible to show that TNFα provokes attenuation at Y-phosphorylation of both FAK (at Y397) and Src (at Y416) proteins (P < 0.05), suggesting a decrease in their activities. The very similar profile was observed when osteoblasts were incubated with conditioned medium from lipopolysaccharide (LPS)-stimulated macrophages, it being significantly different than control (FAK and Src, P < 0.05). Nevertheless, in order to validate these findings, we decided to pre-incubate osteoblasts with anti-TNFα neutralizing antibody (2 μg ml-1) prior exposing to conditioned medium. Importantly, our results revealed that there was a diminution on those conditioned medium effects when the same biological parameters were evaluated (P < 0.05). Moreover, we also showed that TNFα impairs osteoblast adhesion, suggesting an interesting role on osteoblast performance. Conclusions: Altogether, these results suggest that LPS-stimulated macrophage mediators attenuate both FAK and Src activations in osteoblast, suggesting a novel role for TNFα on osteoblast performance.
Editor: Blackwell Publishing Ltd
Rights: fechado
Identifier DOI: 10.1111/odi.12202
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-84911990471&partnerID=40&md5=c3c967b0ddefc3886a74d623293b612c
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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