Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/87108
Type: Artigo de periódico
Title: Metabolic And Inflammatory Proteins Differentially Expressed In Platelets From Unprovoked Deep Vein Thrombosis Patients
Author: Flores-Nascimento M.C.
Kleinfelder-Fontanesi K.
de Araujo T.M.
Forato Anhe G.
Secolin R.
Andrade Orsi F.
de Paula E.V.
Annichino-Bizzacchi J.M.
Abstract: Deep vein thrombosis (DVT) is multi-causal disease associated to high morbidity and mortality due to complications, especially from pulmonary embolism. New factors of relevance on the DVT pathophysiology enhance our understanding of disease mechanisms and can be translated into improved management of patients, prevention of recurrence and development of new therapies. In this context, the precise role of platelets in the pathogenesis of DVT is not completely understood. Our objectives were to acquire and to analyze the whole platelet protein profile of samples from 3 DVT patients and to compare them to results obtained from 1 sibling and 1 neighbor from each patient (in order to minimize genetic and environmental interferences). These patients presented unprovoked and recurrent episodes of proximal DVT as well as a family history of DVT. Platelets were washed, lysed, and the proteins were hydrolyzed by trypsin. Peptides were first separated by HPLC and peptide fractions were further detected by LC-MS/MS. Five proteins was present on patients and absent in all the controls: Apolipoprotein A1 Binding-Protein, Coatomer (zeta1 sub-unit), 17β-hydroxysteroid dehydrogenase type XI, Leukotriene A-4 Hydrolase and Sorbitol Dehydrogenase. The analysis identified proteins that currently are not related to the pathophysiology of DVT, and the persistence of these inflammatory and lipid transportation-related proteins emphasize the relevance of these phenomena on DVT. © 2014 Flores-Nascimento MC, et al.
Editor: OMICS Publishing Group
Rights: aberto
Identifier DOI: 10.4172/jpb.1000298
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-84893628831&partnerID=40&md5=098343eab64478b67249fa7d54b23144
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
2-s2.0-84893628831.pdf889.61 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.