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Type: Artigo de evento
Title: Extraction And Preliminary Characterization Of Bromelain From Curaua (ananas Erectifolius L.b.smith) Purple And White
Author: De Lima e Silva T.A.
Bresolin I.R.A.P.
De Campos Takaki G.M.
Aoyama H.
Garrard I.
Tambourgi E.B.
Abstract: The characterization and determination of kinetic parameters of bromelain extracted from curaua (Ananas erectifolius) white and purple varieties were studied. Optimal pH and temperature conditions for proteolytic activity were determined by azocasein method. The enzyme has shown optimum activity at pH 6.0 for white and 6.0 and 7.0 for purple curaua, and at 40 °C and 60 °C for white curaua and at 40°C for the purple curaua. The lowest result of Km and the highest result of Vmax/Km were found for purple curaua, but the two varieties showed affinity by the substrate, with Km value of 185.18 μmolL-1min, Vmax of 158.73 μmolL-1 and activation energy of 13,903 for white curaua and Km of 192.0 μmolL-1min, Vmax of 188.68 μmolL-1 and activation energy of 14,941 cal/mol for purple curaua. This enzyme showed lower activation energy (26,00028,000 cal/mol) than those of other coexisting proteinases. Copyright © 2014,AIDIC Servizi S.r.l.
Editor: Italian Association of Chemical Engineering - AIDIC
Rights: fechado
Identifier DOI: 10.3303/CET1437129
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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