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|Type:||Artigo de periódico|
|Title:||Expanding Resolution Of Metalloprotein Separations From Soybean Seeds Using 2d-hplc-icp-ms And Sds-page As A Third Dimension|
|Abstract:||This work reports on the use of a three dimensional separation system to enhance metalloprotein information when considering soybean seeds. Separations using size exclusion chromatography (SEC) allowed identification of three metal fractions. Following an anion exchange (AEX) chromatographic separation in the second dimension, the resultant sub-fractions were lyophilized and subjected to a third dimension of separation using a polyacrylamide gel electrophoresis (SDS-PAGE). After the separation, the bands were digested, and, in addition to others, the following proteins, previously associated with metals, were identified: 3-lipoxygenase A chain (soybean) complex with 13(S)-hydroperoxy-9(Z),11(E)-octadecadienoic acid, beta-amylase [. Glycine max], seed lipoxygenase-1, lipoxygenase [. G. max], seed lipoxygenase-2 ( Pisum sativum) and beta-conglycinin. © 2014 Elsevier B.V.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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