Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/86645
Type: Artigo
Title: A New fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells
Author: Gasparotto, Vinícius P. O.
Landim-Alvarenga, Fernanda C.
Oliveira, Alexandre L. R.
Simões, Gustavo Ferreira
Lima-Neto, João F.
Barraviera, Benedito
Ferrira Jr., Rui S.
Abstract: Introduction. The optimization of an organic scaffold for specific types of applications and cells is vital to successful tissue engineering. In this study, we investigated the effects of a new fibrin sealant derived from snake venom as a scaffold for mesenchymal stem cells, to demonstrate the ability of cells to affect and detect the biological microenvironment. Methods. The characterization of CD34, CD44 and CD90 expression on mesenchymal stem cells was performed by flow cytometry. In vitro growth and cell viability were evaluated by light and electron microscopy. Differentiation into osteogenic, adipogenic and chondrogenic lineages was induced. Results: The fibrin sealant did not affect cell adhesion, proliferation or differentiation and allowed the adherence and growth of mesenchymal stem cells on its surface. Hoechst 33342 and propidium iodide staining demonstrated the viability of mesenchymal stem cells in contact with the fibrin sealant and the ability of the biomaterial to maintain cell survival. Conclusions: The new fibrin sealant is a three-dimensional scaffolding candidate that is capable of maintaining cell survival without interfering with differentiation, and might also be useful in drug delivery. Fibrin sealant has a low production cost, does not transmit infectious diseases from human blood and has properties of a suitable scaffold for stem cells because it permits the preparation of differentiated scaffolds that are suitable for every need. © 2014 Gasparotto et al.; licensee BioMed Central Ltd.
The optimization of an organic scaffold for specific types of applications and cells is vital to successful tissue engineering. In this study, we investigated the effects of a new fibrin sealant derived from snake venom as a scaffold for mesenchymal stem
Subject: Selante de fibrina
Venenos de serpentes
Células mesenquimais estromais
Citometria de fluxo
Microscopia eletrônica
Country: Reino Unido
Editor: BioMed Central Ltd.
Citation: Stem Cell Research And Therapy. Biomed Central Ltd., v. 5, n. 3, p. - , 2014.
Rights: aberto
aberto
Identifier DOI: 10.1186/scrt467
Address: https://stemcellres.biomedcentral.com/articles/10.1186/scrt467
Date Issue: 2014
Appears in Collections:IB - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
2-s2.0-84903985284.pdf2.08 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.