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Type: Artigo de periódico
Title: β-glucosidase Immobilisation On Synthetic Superparamagnetic Magnetite Nanoparticles And Their Application In Saccharification Of Wheat Straw And Eucalyptus Globulus Pulps
Author: Valenzuela R.
Castro J.F.
Parra C.
Baeza J.
Duran N.
Freer J.
Abstract: β-Glucosidase from Trichoderma reesei was immobilised on synthetic superparamagnetic magnetite (Fe3O4) nanoparticles with a mean diameter of 10 nm and were used to supplement cellulase in the enzymatic hydrolysis of three substrates: wheat straw pretreated by steam explosion, Eucalyptus globulus pretreated by hydrothermolysis and E. globulus pretreated by hydrothermolysis followed by alkaline extraction. The hydrolysis yields for each pretreated material, using immobilised β-glucosidase (I-βG) and free cellulase, were 76.1%, 83.6% and 75.6%, respectively, and resulted in an improved hydrolysis yields compared with only cellulase. These yields were at most 10% lower than yields reached with free enzymes. The (I-βG) was magnetically recovered and successfully reused twice. The differences in the hydrolysis yields were not significant (p > 0.05) in the case of steam-exploded wheat straw and E. globulus pretreated by hydrothermolysis followed by alkaline extraction. The immobilisation of enzymes provides an opportunity to reduce the costs of enzymes in the bioethanol production process. © 2014 Taylor and Francis Group, LLC.
Rights: fechado
Identifier DOI: 10.1080/17458080.2011.651167
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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