Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/86477
Type: Artigo de periódico
Title: Evaluation Of Cardiovascular Effects Of Edible Fruits Of Syzygium Cumini Myrtaceae (l) Skeels In Rats
Author: Herculano E.A.
da Costa C.D.F.
Rodrigues A.K.B.F.
Araujo-Junior J.X.
Santana A.E.G.
Franca P.H.B.
Gomes E.A.
Salvador M.J.
Moura F.B.P.
Ribeiro E.A.N.
Abstract: Purpose: To evaluate the hypotensive, vasorelaxant and antihypertensive effects elicited by the hydroalcohol extract from the fruits of Syzygium cumini (EHSCF) in non-anesthetized rats.Methods: The rats were anesthetized and polyethylene catheters were inserted into the lower abdominal aorta and into the inferior vena cava for blood pressure measurements and administration of drugs. After a recovery period of 24 h, EHSCF (0.5; 1; 5; 10; 20 and 30 mg/kg, i.v.) was administered in non-anesthetized rats. The mean arterial pressure and the heart rate were recorded. To investigate the effects of extract, doses EHSCF were administered after pretreatment with L-NAME, atropine, indomethacin, and hexamethonium. For measurement of isometric tension, a concentration-response curve was obtained after Phenylephrine and KCl (80 mM) pre-contractions. The bioactive extract was analyzed via mass spectrometry (MS) fingerprinting using direct electrospray ionization mass spectrometry (ESI-MS).Results: EHSCF (0.5; 1; 5; 10; 20  nd 30 mg/kg) induced hypotension (-15 ± 1, -14 ± 1, -15 ± 1, -13 ± 1, -11 ± 1 and -13 ± 2 %) and bradycardia (-6 ± 1, -5 ± 1, -6 ± 1, -14 ± 1, -8 ± 1 and -10 ± 2 %) in normotensive rats. These responses were attenuated by pre-treatment with L-NAME, indomethacin, hexamethonium or atropine. In phenylephrine, pre-contracted mesenteric rings, EHSCF-induced relaxation (Emax = 54.6 ± 4.5 % and pD2 = 2.7 ± 0.1) that were affected by endothelium removal. EHSCF caused relaxant effect of KCl (80 mM) pre-contracted rings (Emax = 100 ± 0.2 % and pD2 = 2.2 ± 0.1). This effect was not changed in denuded rings. A single oral administration of the extract reduced significant mean arterial pressure in spontaneously hypertensive rats. ESI-MS/MS analyses of EHSCF demonstrated that the major constituents of the analyzed samples coincided with the mass of the malic, gallic, caffeic and ferulic acids.Conclusion: The results suggest that EHSCF induces hypotension probably due to a decrease inperipheral resistance, mediated by the  ndothelium. Bradycardia may be due to indirect cardiac muscarinic activation. The extract also causes an antihypertensive effect.
Editor: University of Benin
Rights: fechado
Identifier DOI: 10.4314/tjpr.v13i11.12
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-84912119835&partnerID=40&md5=f44e3ec7bd425e4b2e2fce25d32d6b5d
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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