Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/85878
Type: Artigo de periódico
Title: Fast Method For Capsaicinoids Analysis From Capsicum Chinense Fruits
Author: Sganzerla M.
Coutinho J.P.
de Melo A.M.T.
Godoy H.T.
Abstract: Chili peppers are widely utilized in the world as savory food additives due the pungency induced by the capsaicinoids. Also, these compounds have functional properties as antimutagenic, antitumoral, antioxidant and analgesic. These characteristics increase the interest in this compound class, hence the capsaicinoid analysis must be reproducible and accurate. This study aimed to develop and validate a fast, efficient and reproducible method to analyze capsaicinoids in Brazilian Capsicum chinense fruits. The extracts were obtained after an optimization step that indicated the condition 100% of methanol and 10min on ultrasound assisted extraction. The analyses were carried out in an ultra high performance liquid chromatographic system with detection by a photo diode array and mass spectrometer. The analytical method developed permits the separation of 8 capsaicinoids in 4min of time analysis expending only 2mL of solvent as mobile phase. The validation parameters evaluated for the method show the effectiveness and satisfactory performance to answer the analytical needs of this research area, presenting low values to relative standard deviation in repeatability and reproducibility and recoveries ranged from 88 to 112% for capsaicin and 89 to 109% for dihydrocapsaicin. In the extracts from different accessions of C. chinense fruits analyzed, the contents of capsaicin and dihydrocapsaicin were in the range of 156-1442μgg-1 and 26-478μgg-1 of fresh fruit, respectively, showing the large application of this method for quantification of the two major capsaicinoids in fast routine analysis and may be used to determine the concentrations of other minor capsaicinoids once appropriate standards are available. © 2014 Elsevier Ltd.
Editor: Elsevier Ltd
Rights: fechado
Identifier DOI: 10.1016/j.foodres.2014.08.003
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-84907301150&partnerID=40&md5=2aa2d2939a60aafc5fa6002affa33a5f
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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