Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/85871
Type: Artigo de periódico
Title: The Amazonian Fruit Byrsonima Crassifolia Effectively Scavenges Reactive Oxygen And Nitrogen Species And Protects Human Erythrocytes Against Oxidative Damage
Author: Mariutti L.R.B.
Rodrigues E.
Chiste R.C.
Fernandes E.
Mercadante A.Z.
Abstract: A hydrophilic extract of murici (Byrsonima crassifolia), a fruit native to the North and Northeast regions of Brazil, was evaluated in relation to its phenolic composition and in vitro antioxidant potential against some physiologically relevant reactive oxygen and nitrogen species. Additionally, the protective effect of murici extract against peroxyl radical (ROO<sup/>)-induced toxicity to human erythrocytes was also determined. The major phenolic compound, determined by HPLC-DAD-MSn, was quercetin (2.72 ± 0.35μg/mL). The extract was able to scavenge ROO<sup/> (0.30 ± 0.04μmoltroloxequivalent/mg), hypochlorous acid (IC50=10.0 ± 0.1μg/mL), hydroxyl radical (IC50=7 ± 1μg/mL) and peroxynitrite anion (IC50=21.0 ± 0.6μg/mL and 17.0 ± 1.6μg/mL, respectively, in absence and presence of NaHCO3). Human erythrocytes were subjected to oxidative damage, but murici extract was not able to inhibit hemolysis, even at the highest tested concentration. On the other hand, the extract inhibited hemoglobin oxidation (IC50=271 ± 44μg/mL), lipid peroxidation (1000μg/mL) by 48 ± 5%, depletion of glutathione (100μg/mL) by 49 ± 2% and formation of its oxidized form (100μg/mL) by 96 ± 4%.
Editor: Elsevier Ltd
Rights: fechado
Identifier DOI: 10.1016/j.foodres.2014.07.032
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-84907852522&partnerID=40&md5=636b50fadec03beb4a8d6ccdeeb0ca32
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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